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Literature summary for 1.14.15.18 extracted from

  • Hosseinpour, F.; Norlin, M.; Wikvall, K.
    Kidney microsomal 25- and 1alpha-hydroxylase in vitamin D metabolism: catalytic properties, molecular cloning, cellular localization and expression during development (2002), Biochim. Biophys. Acta, 1580, 133-144.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in COS cells, vitamin D3 25- and 1alpha-hydroxylase, DNA sequence analysis Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome vitamin D3 25- and 1alpha-hydroxylase Sus scrofa
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56370
-
predicted from DNA sequence analysis Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
25-hydroxycholecalciferol + NADPH + O2 Sus scrofa
-
calcitriol + NADP+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
vitamin D3 25- and 1alpha-hydroxylase Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
kidney proximal tubular cells Sus scrofa
-
liver
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
25-hydroxycholecalciferol + NADPH + O2
-
Sus scrofa calcitriol + NADP+ + H2O
-
?
calcidiol + NADPH + O2 enzyme shows vitamin D3 25- and 1alpha-hydroxylase activity Sus scrofa calcitriol + NADP+ + H2O calcitriol is identical with 1,25-dihydroxycholecalciferol ?
calcidiol + NADPH + O2 highly specific for calcidiol, calcidiol is identical with 25-hydroxycholecalciferol Sus scrofa calcitriol + NADP+ + H2O calcitriol is identical with 1,25-dihydroxycholecalciferol ?

Synonyms

Synonyms Comment Organism
CYB27B1
-
Sus scrofa
vitamin D3 25- and 1alpha-hydroxylase i.e. CYP2D25, microsomal, higher 25-hydroxylating than 1alpha-hydroxylating activity Sus scrofa