Cloned (Comment) | Organism |
---|---|
expression in COS cells, vitamin D3 25- and 1alpha-hydroxylase, DNA sequence analysis | Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | vitamin D3 25- and 1alpha-hydroxylase | Sus scrofa | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56370 | - |
predicted from DNA sequence analysis | Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
25-hydroxycholecalciferol + NADPH + O2 | Sus scrofa | - |
calcitriol + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
vitamin D3 25- and 1alpha-hydroxylase | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | proximal tubular cells | Sus scrofa | - |
liver | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
25-hydroxycholecalciferol + NADPH + O2 | - |
Sus scrofa | calcitriol + NADP+ + H2O | - |
? | |
calcidiol + NADPH + O2 | enzyme shows vitamin D3 25- and 1alpha-hydroxylase activity | Sus scrofa | calcitriol + NADP+ + H2O | calcitriol is identical with 1,25-dihydroxycholecalciferol | ? | |
calcidiol + NADPH + O2 | highly specific for calcidiol, calcidiol is identical with 25-hydroxycholecalciferol | Sus scrofa | calcitriol + NADP+ + H2O | calcitriol is identical with 1,25-dihydroxycholecalciferol | ? |
Synonyms | Comment | Organism |
---|---|---|
CYB27B1 | - |
Sus scrofa |
vitamin D3 25- and 1alpha-hydroxylase | i.e. CYP2D25, microsomal, higher 25-hydroxylating than 1alpha-hydroxylating activity | Sus scrofa |