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Literature summary for 1.14.15.18 extracted from
- Properties of guinea-pig kidney 25-hydroxyvitamin D3 1 alpha-hydroxylase assayed by isotope dilution-mass spectrometry (1988), Biochem. J., 250, 521-526.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,25-Dihydroxycholecalciferol | calcitriol | Cavia porcellus |  |
| antimycin A | no inhibition of hydroxylation | Cavia porcellus | |
| CO | - |
Cavia porcellus | |
| cyanide | - |
Cavia porcellus | |
| p-Trifluoromethoxyphenylhydrazone | - |
Cavia porcellus | |
| rotenone | no inhibition of hydroxylation | Cavia porcellus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.001 | - |
25-hydroxycholecalciferol | - |
Cavia porcellus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Cavia porcellus | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 10 mM used in standard incubation | Cavia porcellus | |
| additional information | highest conversion with a hypo-osmolar buffer | Cavia porcellus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 25-hydroxycholecalciferol + NADPH + O2 | Cavia porcellus | energy-dependent transhydrogenation is of importance | calcitriol + NADP+ + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cavia porcellus | - |
- |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| calcidiol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitriol + 2 oxidized adrenodoxin + H2O | 25-hydroxyvitamin D3 1alpha-hydroxylase is a cytochrome P-450-dependent mixed-function oxidase | Cavia porcellus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | - |
Cavia porcellus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 25-hydroxycholecalciferol + NADPH + O2 | energy-dependent transhydrogenation is of importance | Cavia porcellus | calcitriol + NADP+ + H2O | - |
? | |
| calcidiol + NADPH + O2 | highly specific for calcidiol, calcidiol is identical with 25-hydroxycholecalciferol | Cavia porcellus | calcitriol + NADP+ + H2O | calcitriol is identical with 1,25-dihydroxycholecalciferol | ? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | 8.4 | - |
Cavia porcellus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Cavia porcellus | |
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