BRENDA - Enzyme Database show
show all sequences of 1.14.15.15

Isolation of rat liver mitochondrial ferredoxin and its reductase active in the 5beta-cholestane-3alpha,7alpha,12alpha-triol 26 hydroxylase

Atsuta, Y.; Okuda, K.; J. Biol. Chem. 253, 4653-4658 (1978)

Data extracted from this reference:

Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
-
Rattus norvegicus
5739
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rattus norvegicus
-
-
-
Purification (Commentary)
Commentary
Organism
partial purification of a ferredoxin-like iron-sulfur protein and a NADPH-ferredoxin reductase which are functional for 26-hydroxylation when reconstituted with partially purified liver mitochondrial cytochrome P-450
Rattus norvegicus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Rattus norvegicus
-
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Rattus norvegicus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Rattus norvegicus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
-
Rattus norvegicus
5739
-
Purification (Commentary) (protein specific)
Commentary
Organism
partial purification of a ferredoxin-like iron-sulfur protein and a NADPH-ferredoxin reductase which are functional for 26-hydroxylation when reconstituted with partially purified liver mitochondrial cytochrome P-450
Rattus norvegicus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Rattus norvegicus
-
Other publictions for EC 1.14.15.15
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735739
Ehrhardt
Human CYP27A1 catalyzes hydrox ...
Homo sapiens
Biol. Chem.
397
513-518
2016
-
-
1
-
-
-
-
-
1
-
-
-
-
1
-
-
1
-
-
1
-
-
4
-
1
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
4
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
735690
Frank
Cytochrome P450 125A4, the thi ...
Mycolicibacterium smegmatis
Biochemistry
54
6909-6916
2015
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
735969
Fedorova
Synthesis of an endogenous ste ...
Homo sapiens, Rattus norvegicus
Circ. Cardiovasc. Genet.
8
736-745
2015
-
1
2
-
2
-
-
-
2
-
-
-
-
2
-
-
-
-
-
11
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
2
-
-
2
-
-
-
-
-
2
-
-
-
-
-
-
-
-
11
-
-
-
-
-
-
-
-
-
-
-
-
1
6
6
1
-
-
736710
Uno
Identification and analysis of ...
Macaca fascicularis
J. Vet. Med. Sci.
76
1647-1650
2014
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
11
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
11
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
725831
Endo-Umeda
7-Dehydrocholesterol metabolit ...
Homo sapiens
J. Steroid Biochem. Mol. Biol.
140
7-16
2013
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
736561
Voegeli
Evidence for a role of sterol ...
Homo sapiens, Mus musculus
J. Endocrinol.
219
119-129
2013
-
-
-
-
1
-
-
-
2
-
-
2
-
4
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
4
-
1
-
-
-
-
-
2
-
-
2
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
4
4
-
-
-
725428
Charvet
Isolevuglandins and mitochondr ...
Bos taurus
J. Biol. Chem.
286
20413-20422
2011
-
1
-
-
-
-
-
-
1
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
712202
Nozue
Identification of a novel miss ...
Homo sapiens
Intern. Med.
49
1127-1131
2010
-
-
1
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
712677
Bavner
On the mechanism of accumulati ...
Mus musculus
J. Lipid Res.
51
2722-2730
2010
-
-
1
-
3
-
-
-
1
-
-
-
-
4
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
713175
Schneider
A novel mutation in the sterol ...
Homo sapiens
Orphanet J. Rare Dis.
5
27
2010
-
-
1
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
705935
Gilardi
Expression of sterol 27-hydrox ...
Rattus norvegicus
Neuroscience
164
530-540
2009
1
-
1
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
6
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
6
-
-
2
-
-
-
-
-
-
-
-
-
1
3
3
1
-
-
702407
Pettersson
Metabolism of a novel side cha ...
Homo sapiens
Biochim. Biophys. Acta
1781
383-390
2008
-
-
-
-
-
-
-
2
1
-
-
2
-
1
-
-
-
-
-
3
-
-
4
-
1
-
-
2
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
2
1
-
-
2
-
-
-
-
-
3
-
-
4
-
1
-
-
2
1
-
-
-
-
-
-
-
2
2
702408
Tang
Glucocorticoid receptor-mediat ...
Homo sapiens
Biochim. Biophys. Acta
1781
718-723
2008
1
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
703582
Pou
Ritonavir increases CD36, ABCA ...
Homo sapiens
Exp. Biol. Med.
233
1572-1582
2008
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
705461
Pikuleva
Studies of membrane topology o ...
Homo sapiens
Lipids
43
1127-1132
2008
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
671957
Pikuleva
Putative helix F contributes t ...
Homo sapiens
Biochemistry
40
7621-7629
2001
-
-
1
-
10
-
-
1
1
-
-
2
-
1
-
-
1
-
-
-
1
-
4
1
1
-
-
-
1
-
-
2
-
-
-
-
-
1
2
-
10
-
-
-
-
1
1
-
-
2
-
-
-
1
-
-
1
-
4
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
671685
Furster
Biochemical characterization o ...
Oryctolagus cuniculus
Biochem. Biophys. Res. Commun.
263
663-666
1999
1
-
-
-
-
-
1
-
2
-
2
1
-
2
-
1
1
-
-
6
1
-
6
2
-
-
-
-
1
-
-
2
-
-
-
1
-
-
2
-
-
-
-
1
-
-
2
-
2
1
-
-
1
1
-
6
1
-
6
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
674402
Holmberg-Betsholtz
-
Sterol 27-hydroxylase in bile ...
Oryctolagus cuniculus
J. Biol. Chem.
266
11079-11085
1993
-
-
-
-
-
-
-
-
-
-
-
3
-
1
-
-
-
1
-
-
-
-
5
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
5
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
439034
Ohyama
Multi-functional property of r ...
Rattus norvegicus
J. Biochem.
109
389-393
1991
-
-
-
-
-
-
3
-
1
-
-
-
-
1
-
-
1
-
-
1
2
-
3
-
-
-
1
3
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
3
1
-
1
-
-
-
-
-
-
1
-
1
2
-
3
-
-
-
1
3
-
-
-
-
-
-
-
-
-
-
439035
Akiyoshi-Shibata
Expression of rat liver vitami ...
Rattus norvegicus
FEBS Lett.
280
367-370
1991
-
-
1
-
-
-
-
-
4
-
-
-
-
5
-
-
-
-
-
4
-
-
2
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
4
-
-
2
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
439036
Usui
Unique property of liver mitoc ...
Rattus norvegicus
FEBS Lett.
274
175-177
1990
-
-
1
-
-
-
-
-
1
-
1
-
-
1
-
-
1
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
1
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
674400
Andemson
Cloning, structure, and expres ...
Oryctolagus cuniculus
J. Biol. Chem.
264
8222-8229
1989
-
-
1
-
-
-
-
-
1
-
1
6
-
3
-
-
1
-
-
1
-
-
6
1
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
-
1
-
1
6
-
-
-
1
-
1
-
-
6
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439030
Okuda
Purification and characterizat ...
Rattus norvegicus
J. Biol. Chem.
263
18138-18142
1988
-
-
-
-
-
-
4
1
1
-
1
-
-
1
-
-
1
-
-
1
1
-
3
-
-
-
-
3
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
4
-
1
1
-
1
-
-
-
-
1
-
1
1
-
3
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
439031
Thompson
Evidence for peroxisomal hydro ...
Rattus norvegicus
Biochem. Biophys. Res. Commun.
130
708-716
1985
-
-
-
-
-
-
-
-
3
1
-
1
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
3
1
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
674399
Wikvall
Hydroxylations in biosynthesis ...
Oryctolagus cuniculus
J. Biol. Chem.
259
3800-3804
1984
-
-
-
-
-
-
-
-
1
-
-
5
-
1
-
-
-
-
-
1
-
-
6
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
-
5
-
-
-
-
-
1
-
-
6
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
389410
Okuda
Physiological function and kin ...
Homo sapiens
J. Biol. Chem.
258
2899-2905
1983
-
-
-
-
-
-
3
-
-
-
-
1
-
1
-
-
1
-
-
1
1
-
2
-
-
-
-
-
1
1
-
1
1
-
-
-
-
-
1
-
-
-
-
3
1
-
-
-
-
1
-
-
-
1
-
1
1
-
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
439029
Atsuta
Partial purification and chara ...
Rattus norvegicus
J. Lipid Res.
23
345-351
1982
-
-
-
-
-
-
6
2
1
-
-
-
-
1
-
-
1
-
-
1
-
-
3
-
-
-
-
-
1
1
-
-
1
-
-
-
-
-
-
-
-
-
-
6
1
2
1
-
-
-
-
-
-
1
-
1
-
-
3
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
439032
Hansson
25-Hydroxylation vitamin D3 an ...
Oryctolagus cuniculus, Rattus norvegicus
J. Biol. Chem.
256
4345-4349
1981
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439033
Atsuta
Isolation of rat liver mitocho ...
Rattus norvegicus
J. Biol. Chem.
253
4653-4658
1978
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439025
Okuda
Photochemical action spectrum ...
Rattus norvegicus
Biochem. Biophys. Res. Commun.
74
1071-1076
1977
-
-
-
-
-
-
1
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439026
Okuda
Spectral evidence for a liver ...
Rattus norvegicus
Hoppe-Seyler's Z. Physiol. Chem.
358
689-694
1977
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439028
Sato
Hepatic mitochondrial cytochro ...
Rattus norvegicus
Proc. Natl. Acad. Sci. USA
74
5477-5481
1977
-
-
-
-
-
-
1
-
1
-
-
-
-
1
-
-
1
-
-
1
-
1
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439024
Taniguchi
Enzymatic characteristics of C ...
Rattus norvegicus
Eur. J. Biochem.
40
607-617
1973
-
-
-
-
-
-
4
2
2
-
-
1
-
1
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
4
-
2
2
-
-
1
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288209
Okuda
Separation of 5-beta-cholestan ...
Rattus norvegicus
Biochem. Biophys. Res. Commun.
33
788-793
1968
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439023
Okuda
Oxidation of 5-beta-cholestane ...
Mus musculus, Rattus norvegicus
Biochim. Biophys. Acta
164
381-388
1968
-
-
-
-
-
-
-
1
1
-
-
1
-
2
-
-
-
-
-
1
-
-
3
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
-
1
-
-
-
-
-
1
-
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
288207
Masui
The oxidation of 5-beta-choles ...
Rattus norvegicus
Biochim. Biophys. Acta
117
266-268
1966
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
1
-
-
1
-
1
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-