BRENDA - Enzyme Database show
show all sequences of 1.14.15.11

Hydroxylation of Compactin (ML-236B) by CYP105D7 (SAV_7469) from Streptomyces avermitilis

Yao, Q.; Ma, L.; Liu, L.; Ikeda, H.; Fushinobu, S.; Li, S.; Xu, L.H.; J. Microbiol. Biotechnol. 27, 956-964 (2017)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escheirchia coli
Streptomyces avermitilis
Crystallization (Commentary)
Crystallization
Organism
molecular docking of compactin, compactin is directly hydrogen bonded with residues Thr79, Arg81, Leu178, Thr391, and Ile392 and its C6 position is proximate to the heme group. Molecular docking of diclofenac
Streptomyces avermitilis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.039
-
Compactin
pH 7.4, 30°C
Streptomyces avermitilis
0.19
-
diclofenac
pH 7.4, 30°C
Streptomyces avermitilis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Streptomyces avermitilis
Q825I8
-
-
Streptomyces avermitilis DSM 46492
Q825I8
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
compactin + reduced ferredoxin + O2
-
745541
Streptomyces avermitilis
pravastatin + oxidized ferredoxin + H2O
-
-
-
?
compactin + reduced ferredoxin + O2
-
745541
Streptomyces avermitilis DSM 46492
pravastatin + oxidized ferredoxin + H2O
-
-
-
?
compactin + reduced putidaredoxin + O2
-
745541
Streptomyces avermitilis
pravastatin + oxidized putidaredoxin + H2O
-
-
-
?
compactin + reduced putidaredoxin + O2
-
745541
Streptomyces avermitilis DSM 46492
pravastatin + oxidized putidaredoxin + H2O
-
-
-
?
diclofenac + reduced putidaredoxin + O2
-
745541
Streptomyces avermitilis
4'-hydroxydiclofenac + oxidized putidaredoxin + H2O
-
-
-
?
diclofenac + reduced putidaredoxin + O2
-
745541
Streptomyces avermitilis DSM 46492
4'-hydroxydiclofenac + oxidized putidaredoxin + H2O
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 45000, SDS-PAGE, recombinant protein
Streptomyces avermitilis
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.018
-
Compactin
pH 7.4, 30°C
Streptomyces avermitilis
0.36
-
diclofenac
pH 7.4, 30°C
Streptomyces avermitilis
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escheirchia coli
Streptomyces avermitilis
Crystallization (Commentary) (protein specific)
Crystallization
Organism
molecular docking of compactin, compactin is directly hydrogen bonded with residues Thr79, Arg81, Leu178, Thr391, and Ile392 and its C6 position is proximate to the heme group. Molecular docking of diclofenac
Streptomyces avermitilis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.039
-
Compactin
pH 7.4, 30°C
Streptomyces avermitilis
0.19
-
diclofenac
pH 7.4, 30°C
Streptomyces avermitilis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
compactin + reduced ferredoxin + O2
-
745541
Streptomyces avermitilis
pravastatin + oxidized ferredoxin + H2O
-
-
-
?
compactin + reduced ferredoxin + O2
-
745541
Streptomyces avermitilis DSM 46492
pravastatin + oxidized ferredoxin + H2O
-
-
-
?
compactin + reduced putidaredoxin + O2
-
745541
Streptomyces avermitilis
pravastatin + oxidized putidaredoxin + H2O
-
-
-
?
compactin + reduced putidaredoxin + O2
-
745541
Streptomyces avermitilis DSM 46492
pravastatin + oxidized putidaredoxin + H2O
-
-
-
?
diclofenac + reduced putidaredoxin + O2
-
745541
Streptomyces avermitilis
4'-hydroxydiclofenac + oxidized putidaredoxin + H2O
-
-
-
?
diclofenac + reduced putidaredoxin + O2
-
745541
Streptomyces avermitilis DSM 46492
4'-hydroxydiclofenac + oxidized putidaredoxin + H2O
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 45000, SDS-PAGE, recombinant protein
Streptomyces avermitilis
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.018
-
Compactin
pH 7.4, 30°C
Streptomyces avermitilis
0.36
-
diclofenac
pH 7.4, 30°C
Streptomyces avermitilis
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.48
-
Compactin
pH 7.4, 30°C
Streptomyces avermitilis
1.9
-
diclofenac
pH 7.4, 30°C
Streptomyces avermitilis
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.48
-
Compactin
pH 7.4, 30°C
Streptomyces avermitilis
1.9
-
diclofenac
pH 7.4, 30°C
Streptomyces avermitilis
Other publictions for EC 1.14.15.11
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745541
Yao
Hydroxylation of Compactin (M ...
Streptomyces avermitilis, Streptomyces avermitilis DSM 46492
J. Microbiol. Biotechnol.
27
956-964
2017
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1
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6
1
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2
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2
2
744146
Xu
Structural basis for the 4'-h ...
Streptomyces avermitilis, Streptomyces avermitilis DSM 46492
Appl. Microbiol. Biotechnol.
99
3081-3091
2015
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1
1
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-
1
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5
-
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4
1
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1
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1
1
1
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1
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4
1
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719674
Takamatsu
Pentalenic acid is a shunt met ...
Streptomyces avermitilis
J. Antibiot.
64
65-71
2011
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1
-
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1
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4
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2
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1
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1
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1
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2
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1
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1
1
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-
719135
Pandey
Regioselective hydroxylation o ...
Streptomyces avermitilis, Streptomyces avermitilis MA-4680
Biotechnol. Bioeng.
105
697-704
2010
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5
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2
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