BRENDA - Enzyme Database
show all sequences of 1.14.15.11

Pentalenic acid is a shunt metabolite in the biosynthesis of the pentalenolactone family of metabolites: hydroxylation of 1-deoxypentalenic acid mediated by CYP105D7 (SAV_7469) of Streptomyces avermitilis

Takamatsu, S.; Xu, L.H.; Fushinobu, S.; Shoun, H.; Komatsu, M.; Cane, D.E.; Ikeda, H.; J. Antibiot. 64, 65-71 (2011)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli
Streptomyces avermitilis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.027
-
1-deoxypentalenic acid
pH 8.0, 25C
Streptomyces avermitilis
Organism
Organism
UniProt
Commentary
Textmining
Streptomyces avermitilis
Q825I8
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1-deoxypentalenic acid + reduced ferredoxin + O2
-
719674
Streptomyces avermitilis
pentalenic acid + oxidized ferredoxin + H2O
-
-
-
?
additional information
no substrates: pentalenene, 1-deoxy-11beta-hydroxypentalenic acid, 1-deoxy-11-oxopentalenic acid, or the corresponding methyl esters of 1-deoxy-11beta-hydroxypentalenic acid, 1-deoxy-11-oxopentalenic acid and 1-deoxypentalenic acid, respectively
719674
Streptomyces avermitilis
?
-
-
-
-
Synonyms
Synonyms
Commentary
Organism
CYP105D7
-
Streptomyces avermitilis
CYP28
-
Streptomyces avermitilis
SAV_7469
locus name
Streptomyces avermitilis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Streptomyces avermitilis
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Streptomyces avermitilis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.027
-
1-deoxypentalenic acid
pH 8.0, 25C
Streptomyces avermitilis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1-deoxypentalenic acid + reduced ferredoxin + O2
-
719674
Streptomyces avermitilis
pentalenic acid + oxidized ferredoxin + H2O
-
-
-
?
additional information
no substrates: pentalenene, 1-deoxy-11beta-hydroxypentalenic acid, 1-deoxy-11-oxopentalenic acid, or the corresponding methyl esters of 1-deoxy-11beta-hydroxypentalenic acid, 1-deoxy-11-oxopentalenic acid and 1-deoxypentalenic acid, respectively
719674
Streptomyces avermitilis
?
-
-
-
-
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Streptomyces avermitilis
General Information
General Information
Commentary
Organism
physiological function
disruption of the sav7469 gene encoding pentalenic acid synthase abolishes production of pentalenic acid. The deletion mutant accumulates 1-deoxypentalenic acid. Recombinant enzyme prepared from Escherichia coli catalyzes the oxidative conversion of 1-deoxypentalenic acid to pentalenic acid in the presence of the electron-transport partners, ferredoxin and ferredoxin reductase, both in vivo and in vitro
Streptomyces avermitilis
General Information (protein specific)
General Information
Commentary
Organism
physiological function
disruption of the sav7469 gene encoding pentalenic acid synthase abolishes production of pentalenic acid. The deletion mutant accumulates 1-deoxypentalenic acid. Recombinant enzyme prepared from Escherichia coli catalyzes the oxidative conversion of 1-deoxypentalenic acid to pentalenic acid in the presence of the electron-transport partners, ferredoxin and ferredoxin reductase, both in vivo and in vitro
Streptomyces avermitilis
Other publictions for EC 1.14.15.11
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745541
Yao
Hydroxylation of Compactin (M ...
Streptomyces avermitilis, Streptomyces avermitilis DSM 46492
J. Microbiol. Biotechnol.
27
956-964
2017
-
-
1
1
-
-
-
2
-
-
-
-
-
4
-
-
-
-
-
-
-
-
6
1
2
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
6
1
-
-
-
2
-
-
-
-
-
-
-
-
2
2
744146
Xu
Structural basis for the 4'-h ...
Streptomyces avermitilis, Streptomyces avermitilis DSM 46492
Appl. Microbiol. Biotechnol.
99
3081-3091
2015
-
-
1
1
-
-
-
1
-
-
-
-
-
5
-
-
-
-
-
-
-
-
4
1
2
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
719674
Takamatsu
Pentalenic acid is a shunt met ...
Streptomyces avermitilis
J. Antibiot.
64
65-71
2011
-
-
1
-
-
-
-
1
-
-
-
-
-
4
-
-
-
-
-
-
-
-
2
-
3
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
1
1
-
-
-
719135
Pandey
Regioselective hydroxylation o ...
Streptomyces avermitilis, Streptomyces avermitilis MA-4680
Biotechnol. Bioeng.
105
697-704
2010
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-