Cloned (Comment) | Organism |
---|---|
expression of P450cam C334A in Escherichia coli NCM533 | Pseudomonas putida |
Protein Variants | Comment | Organism |
---|---|---|
C334A | the mutation of P450cam increases the protein stability compared to the wild-type enzyme | Pseudomonas putida |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Pseudomonas putida | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | heme protein | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
1 * 46000 | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | P00183 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant P450cam C334A from Escherichia coli NCM533. Protein purification includes a protamine sulfate cut to precipitate nucleic acids, and an ammonium sulfate cut to isolate CYP101A1 | Pseudomonas putida |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 46000 | Pseudomonas putida |
More | three-dimensional structure and dynamics of the open and closed states of CYP101A1, NMR analysis and molecular dynamics simulations, overview | Pseudomonas putida |
Synonyms | Comment | Organism |
---|---|---|
camphor monooxygenase | - |
Pseudomonas putida |
CYP101A1 | - |
Pseudomonas putida |
cytochrome p450cam | - |
Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Pseudomonas putida |
General Information | Comment | Organism |
---|---|---|
additional information | CYP101A1 is a soluble monomeric heme-containing camphor monooxygenase | Pseudomonas putida |