Application | Comment | Organism |
---|---|---|
biotechnology | bioengineered Escherichia coli cells possess a heterologous self-sufficient P450 catalytic system that may have advantages in terms of low cost and high yield for the production of fine chemicals | Pseudomonas putida |
Cloned (Comment) | Organism |
---|---|
functional expression in Escherichia coli DH5alpha of tricistronic constructs consisting of P450cam encoded by the first cistron and the auxiliary proteins, putidaredoxin and putidaredoxin reductase by the second and the third | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
x * 47000, SDS-PAGE/Coomassie staining | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(1R)-(+)-camphor + O2 + reduced putidaredoxin | - |
Pseudomonas putida | (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O | - |
? | |
(4S)-limonene + putidaredoxin + O2 | the 7-position is the major site of hydroxylation by P450cam | Pseudomonas putida | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 47000, SDS-PAGE/Coomassie staining | Pseudomonas putida |
Synonyms | Comment | Organism |
---|---|---|
cytochrome p450cam | - |
Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
putidaredoxin | - |
Pseudomonas putida |