Inhibitors | Comment | Organism | Structure |
---|---|---|---|
putidaredoxin | - |
Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km-value of putidaredoxin, 0.0038 mM, steady-state kinetics of enzyme interaction with redox partners | Pseudomonas putida | |
0.077 | - |
O2 | recombinant enzyme with His6-tag, pH 7.4, 25°C | Pseudomonas putida | |
0.083 | - |
O2 | wild-type, pH 7.4, 25°C | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(+)-camphor + reduced putidaredoxin + O2 = (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O | putidaredoxin acts as a shuttle for transport of electrons from putidaredoxin reductase to enzyme | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-camphor + putidaredoxin + O2 | - |
Pseudomonas putida | (R)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
55 | - |
O2 | recombinant enzyme with His6-tag, pH 7.4, 25°C | Pseudomonas putida | |
66 | - |
O2 | wild-type, pH 7.4, 25°C | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P450 | - |
Pseudomonas putida |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00038 | - |
putidaredoxin | recombinant enzyme with His6-tag, pH 7.4, 25°C | Pseudomonas putida | |
0.0007 | - |
putidaredoxin | wild-type, pH 7.4, 25°C | Pseudomonas putida |