Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-nicotine + O2 + [reduced NADPH-hemoprotein reductase] + O2 | Nicotiana tabacum | isoforms CYP82E4, CYP82E5v2, and CYP82E10 demethylate (R)-nicotine 3, 10, and 10fold faster than (S)-nicotine, respectively | (R)-nornicotine + formaldehyde + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
(S)-nicotine + [reduced NADPH-hemoprotein reductase] + O2 | Nicotiana tabacum | only isoform CYP82E4 can significantly convert (S)-nicotine to (S)-nornicotine | (S)-nornicotine + formaldehyde + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana tabacum | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Nicotiana tabacum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-nicotine + O2 + [reduced NADPH-hemoprotein reductase] + O2 | isoforms CYP82E4, CYP82E5v2, and CYP82E10 demethylate (R)-nicotine 3, 10, and 10fold faster than (S)-nicotine, respectively | Nicotiana tabacum | (R)-nornicotine + formaldehyde + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
(S)-nicotine + [reduced NADPH-hemoprotein reductase] + O2 | only isoform CYP82E4 can significantly convert (S)-nicotine to (S)-nornicotine | Nicotiana tabacum | (S)-nornicotine + formaldehyde + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYP82E10 | isoform | Nicotiana tabacum |
CYP82E4 | isoform | Nicotiana tabacum |
CYP82E5v2 | isoform | Nicotiana tabacum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P450 | - |
Nicotiana tabacum | |
NADPH | - |
Nicotiana tabacum |