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Literature summary for 1.14.14.B11 extracted from
- Biochemical and molecular characterizations of nicotine demethylase in tobacco (2007), Physiol. Plant., 129, 307-319.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| D121-AA8 cDNA transformed into yeast cells | Nicotiana tabacum |
| expression in yeast | Nicotiana tabacum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1-aminobenzotriazole | at 1 mM inhibits by 17% | Nicotiana tabacum |  |
| Chloropromazine | 1 mM, 87% inhibition of recombinant nicotine demethylase activity in yeast microsomes | Nicotiana tabacum | |
| chlorpromazine | at 1 mM inhibits by 87% | Nicotiana tabacum | |
| CO | partial reverse activity after light exposure | Nicotiana tabacum | |
| cytochrome c | 1 mM, 78% inhibition of recombinant nicotine demethylase activity in yeast microsomes | Nicotiana tabacum | |
| oxidized cytochrome c | at 0.1 mM inhibits by 78% | Nicotiana tabacum | |
| Piperonylbutoxide | at 1 mM inhibits by 42% | Nicotiana tabacum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0039 | - |
Nicotine | - |
Nicotiana tabacum | |
| 0.0039 | - |
Nicotine | at 25°C, pH 6.25 | Nicotiana tabacum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | putative N-terminal hydrophobic trans-membrane domain | Nicotiana tabacum | 16020 | - |
| microsome | - |
Nicotiana tabacum | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | cysteine-containing heme-binding region | Nicotiana tabacum | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nicotine + NADPH + O2 + H+ | Nicotiana tabacum | demethylates nicotine to form nornicotine, a precursor to the nitrosamine N'-nitrosonornicotine | nornicotine + formaldehyde + NADP+ + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Nicotiana tabacum | Q38Q87 | - |
- |
| Nicotiana tabacum | Q38Q87 | var. 4407 | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Nicotiana tabacum | - |
| leaf | induced in leaves treated with the growth regulator ethylene | Nicotiana tabacum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nicotine + NADPH + O2 + H+ | - |
Nicotiana tabacum | nornicotine + formaldehyde + NADP+ + H2O | - |
? | |
| nicotine + NADPH + O2 + H+ | demethylates nicotine to form nornicotine, a precursor to the nitrosamine N'-nitrosonornicotine | Nicotiana tabacum | nornicotine + formaldehyde + NADP+ + H2O | - |
? | |
| nicotine + NADPH + O2 + H+ | six putative substrate recognition sites | Nicotiana tabacum | nornicotine + formaldehyde + NADP+ + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CYP82E4 | - |
Nicotiana tabacum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome P450 | - |
Nicotiana tabacum | |
| NADPH | - |
Nicotiana tabacum | |
| NADPH | is required for full activity | Nicotiana tabacum | |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Nicotiana tabacum | ethylene induces microsomal demethylase activity in leaves | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | only microsomes from yeast expressing the D121-AA8 cDNA exhibit significant nicotine demethylase activity | Nicotiana tabacum |
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