Literature summary for 1.14.14.B11 extracted from

Yamanaka, H.; Nakajima, M.; Fukami, T.; Sakai, H.; Nakamura, A.; Katoh, M.; Takamiya, M.; Aoki, Y.; Yokoi, T.
CYP2A6 AND CYP2B6 are involved in nornicotine formation from nicotine in humans: interindividual differences in these contributions (2005), Drug Metab. Dispos., 33, 1811-1818.

Search for more literature for 1.14.14.B11

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
anti-CYP2A6 antibody	-	Homo sapiens
coumarin	-	Homo sapiens
additional information	not inhibitory: quinidine, anti-CYP2D6 serum, ketoconazole, anti-CYP3A4 serum	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.049	-	Nicotine	isoform CYP2A6, pH 7.4, 37°C	Homo sapiens
0.55	-	Nicotine	isoform CYP2B6, pH 7.4, 37°C	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
microsome	nicotine N-demethylase activity in microsomes from human livers at 0.02 mM nicotine is significantly correlated with the isoform CYP2A6 contents, while the nicotine N-demethylase activity at 0.1 mM nicotine is significantly correlated with the isoform CYP2B6 contents	Homo sapiens	-	-

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
liver	nicotine N-demethylase activity in microsomes from human livers at 0.02 mM nicotine is significantly correlated with the isoform CYP2A6 contents, while the nicotine N-demethylase activity at 0.1 mM nicotine is significantly correlated with the isoform CYP2B6 contents	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	isoforms CYP2A6 and CYP2B6 significantly contribute to the nicotine N-demethylation at low and high substrate concentrations, respectively	Homo sapiens	?	-	?
nicotine + [reduced NADPH-hemoprotein reductase] + O2	-	Homo sapiens	nornicotine + formaldehyde + [oxidized NADPH-hemoprotein reductase] + H2O	-	?

Cofactor

Cofactor	Comment	Organism	Structure
cytochrome P450	-	Homo sapiens

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Release 2023.1 (January 2023)