Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
anti-CYP2A6 antibody | - |
Homo sapiens | |
coumarin | - |
Homo sapiens | |
additional information | not inhibitory: quinidine, anti-CYP2D6 serum, ketoconazole, anti-CYP3A4 serum | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.049 | - |
Nicotine | isoform CYP2A6, pH 7.4, 37°C | Homo sapiens | |
0.55 | - |
Nicotine | isoform CYP2B6, pH 7.4, 37°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | nicotine N-demethylase activity in microsomes from human livers at 0.02 mM nicotine is significantly correlated with the isoform CYP2A6 contents, while the nicotine N-demethylase activity at 0.1 mM nicotine is significantly correlated with the isoform CYP2B6 contents | Homo sapiens | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | nicotine N-demethylase activity in microsomes from human livers at 0.02 mM nicotine is significantly correlated with the isoform CYP2A6 contents, while the nicotine N-demethylase activity at 0.1 mM nicotine is significantly correlated with the isoform CYP2B6 contents | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | isoforms CYP2A6 and CYP2B6 significantly contribute to the nicotine N-demethylation at low and high substrate concentrations, respectively | Homo sapiens | ? | - |
? | |
nicotine + [reduced NADPH-hemoprotein reductase] + O2 | - |
Homo sapiens | nornicotine + formaldehyde + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P450 | - |
Homo sapiens |