Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme shows significantly enhanced activity upon RAD52 over-expression and cultivation optimization. (-)-Limonene hydroxylation is 2fold increased by RAD52 overexpression in strains PpPM17/AtCPR, expressing limonene-3-hydroxylase (PM17 isoform) and Arabidopsis thaliana cytochrome P450 reductase (CPR), while cultivation at pH 8 yields in about 55% increased isopiperitenol formation. The two effects are largely additive leading to an about 2.5fold enhanced isopiperitenol production in PpPM17/CPR-RAD52 over the starting strain/condition | Mentha x piperita |
Cloned (Comment) | Organism |
---|---|
overexpression in Pichia pastoris. The enzyme shows significantly enhanced activity upon RAD52 over-expression and cultivation optimization. (-)-Limonene hydroxylation is 2fold increased by RAD52 overexpression in strains PpPM17/AtCPR, expressing limonene-3-hydroxylase (PM17 isoform) and Arabidopsis thaliana cytochrome P450 reductase (CPR), while cultivation at pH 8 yields in about 55% increased isopiperitenol formation. The two effects are largely additive leading to an about 2.5fold enhanced isopiperitenol production in PpPM17/CPR-RAD52 over the starting strain/condition | Mentha x piperita |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mentha x piperita | Q9XHE7 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-limonene + [reduced NADPH-hemoprotein reductase] + O2 | - |
Mentha x piperita | (-)-trans-isopiperitenol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
(-)-limonene-3-hydroxylase | - |
Mentha x piperita |
PM17 | - |
Mentha x piperita |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P-450 | a heme-thiolate protein (P-450) | Mentha x piperita | |
NADPH-hemoprotein reductase | A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._ | Mentha x piperita |