Any feedback?
Literature summary for 1.14.14.99 extracted from
- A candidate cDNA clone for (-)-limonene-7-hydroxylase from Perilla frutescens (2010), Phytochemistry, 71, 373-379.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of the chimeric protein in Escherichia coli | Perilla frutescens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of a chimeric mutant enzyme by fusion of the N-terminal membrane insertion domain of the limonene-3-hydroxylase into limonene 7-hydroxylase. The kinetically competent recombinant protein produces a mixture of C3-, C6- and C7-hydroxylated limonene derivatives with a distribution of 33%, 14% and 53%, respectively | Perilla frutescens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-limonene + [reduced NADPH-hemoprotein reductase] + O2 | Perilla frutescens | - |
(-)-trans-isopiperitenol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Perilla frutescens | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-limonene + [reduced NADPH-hemoprotein reductase] + O2 | - |
Perilla frutescens | (-)-trans-isopiperitenol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| additional information | the kinetically competent recombinant protein produces a mixture of C3-, C6- and C7-hydroxylated limonene derivatives, i.e. (-)-perillyl alcohol, (-)-trans-isopiperitenol, and (-)-trans-carveol, with a distribution of 33%, 14% and 53%, respectively, thus it performs the reaction of EC 1.14.13.47 and 1.14.13.48, overview | Perilla frutescens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| (-)-limonene-3-hydroxylase | - |
Perilla frutescens |
| More | the enzyme is a cytochrome P450 limonene hydroxylase | Perilla frutescens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
recombinant chimeric mutant enzyme | Perilla frutescens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.75 | 8.5 | half-maximal activity at pH 6.75 and 8.5, recombinant chimeric mutant enzyme | Perilla frutescens |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
