BRENDA - Enzyme Database show
show all sequences of 1.14.14.99

Hydroxylation of limonene enantiomers and analogs by recombinant (-)-limonene 3- and 6-hydroxylases from Mint (Mentha) species: Evidence for catalysis within sterically constrained active sites

Wust, M.; Little, D.B.; Schalk, M.; Croteau, R.; Arch. Biochem. Biophys. 387, 125-136 (2001)

Data extracted from this reference:

Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mentha x piperita
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(+)-limonene + [reduced NADPH-hemoprotein reductase] + O2
-
390089
Mentha x piperita
(+)-trans-isopiperitenol + [oxidized NADPH-hemoprotein reductase] + H2O
-
390089
Mentha x piperita
-
(-)-(4S)-limonene + [reduced NADPH-hemoprotein reductase] + O2
highly specific, regiospecific and stereospecific reaction
390089
Mentha x piperita
(-)-trans-(3S,4R)-isopiperitenol + [oxidized NADPH-hemoprotein reductase] + H2O
-
390089
Mentha x piperita
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(+)-limonene + [reduced NADPH-hemoprotein reductase] + O2
-
390089
Mentha x piperita
(+)-trans-isopiperitenol + [oxidized NADPH-hemoprotein reductase] + H2O
-
390089
Mentha x piperita
-
(-)-(4S)-limonene + [reduced NADPH-hemoprotein reductase] + O2
highly specific, regiospecific and stereospecific reaction
390089
Mentha x piperita
(-)-trans-(3S,4R)-isopiperitenol + [oxidized NADPH-hemoprotein reductase] + H2O
-
390089
Mentha x piperita
-
Other publictions for EC 1.14.14.99
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745019
Wriessnegger
Enhancing cytochrome P450-med ...
Mentha x piperita
Fungal Genet. Biol.
89
114-125
2016
1
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
1
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
712853
Dolzhenko
UV-B modulates the interplay b ...
Mentha x piperita
J. Photochem. Photobiol. B Biol.
100
67-75
2010
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
1
-
-
1
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-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
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-
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-
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1
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-
-
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1
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
713236
Mau
A candidate cDNA clone for (-) ...
Perilla frutescens
Phytochemistry
71
373-379
2010
-
-
1
-
1
-
-
-
-
-
-
1
-
6
-
-
-
-
-
-
-
-
2
-
-
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-
-
1
1
-
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1
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1
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-
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1
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-
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2
-
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-
-
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1
1
-
-
-
-
-
-
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-
660103
Mahmoud
Cosuppression of limonene-3-hy ...
Mentha x piperita
Phytochemistry
65
547-554
2004
-
1
-
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
2
-
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2
-
-
-
-
-
-
-
-
-
-
-
-
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1
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-
-
-
-
-
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1
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-
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-
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2
-
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2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
660093
Bertea
Molecular evaluation of a spea ...
Mentha x gracilis
Phytochemistry
64
1203-1211
2003
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390089
Wust
Hydroxylation of limonene enan ...
Mentha x piperita
Arch. Biochem. Biophys.
387
125-136
2001
-
-
-
-
-
-
-
-
-
-
-
-
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1
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2
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-
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-
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-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390086
Schalk
A single amino acid substituti ...
Mentha spicata
Proc. Natl. Acad. Sci. USA
97
11948-11953
2000
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
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1
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-
-
-
-
-
-
-
-
-
-
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-
390088
Haudenschild
Functional expression of regio ...
Mentha x piperita
Arch. Biochem. Biophys.
379
127-136
2000
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
1
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-
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-
-
1
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-
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-
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1
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-
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-
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-
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1
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-
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1
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-
-
-
-
-
-
-
-
-
-
-
-
-
390087
Lupien
Regiospecific cytochrome P450 ...
Mentha x piperita
Arch. Biochem. Biophys.
368
181-192
1999
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
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-
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1
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-
390085
Gershenzon
Isolation of secretory cells f ...
Mentha x piperita
Anal. Biochem.
200
130-138
1992
-
-
-
-
-
3
-
-
1
-
-
-
-
1
-
-
1
-
-
1
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
3
-
-
-
-
1
-
-
-
-
-
-
1
-
1
1
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1
-
-
-
-
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1
-
-
-
-
-
-
-
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-
390084
Croteau
Biochemical characterization o ...
Mentha x gracilis, Mentha x piperita
Plant Physiol.
96
744-752
1991
-
-
-
-
-
-
-
-
2
-
-
-
-
2
-
-
-
-
-
2
-
-
8
-
-
-
-
-
-
-
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2
-
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-
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2
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2
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2
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8
-
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-
-
-
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390083
Karp
Monoterpene biosynthesis: spec ...
Mentha x piperita
Arch. Biochem. Biophys.
276
219-226
1990
2
-
-
-
-
-
9
1
1
-
-
1
-
1
-
-
-
1
-
1
1
-
6
-
1
-
-
-
1
1
-
1
-
-
-
2
-
-
1
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-
-
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9
-
1
1
-
-
1
-
-
-
-
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1
1
-
6
-
1
-
-
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1
1
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