BRENDA - Enzyme Database
show all sequences of 1.14.14.98

Elicitor induction and characterization of microsomal protopine-6-hydroxylase, the central enzyme in benzophenanthridine alkaloid biosynthesis

Tanahashi, T.; Zenk, M.H.; Phytochemistry 29, 1113-1122 (1990)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
CO
-
Eschscholzia californica
cytochrome c
-
Eschscholzia californica
ketoconazole
-
Eschscholzia californica
Metyrapone
-
Eschscholzia californica
Prochloraz
-
Eschscholzia californica
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.002
-
protopine
-
Eschscholzia californica
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Chelidonium majus
-
-
microsome
-
Dicentra cucullaria
-
-
microsome
membrane-bound
Eschscholzia californica
-
-
microsome
-
Eschscholzia lobbii
-
-
microsome
-
Fumaria parviflora
-
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
additional information
Eschscholzia californica
the enzyme is specifically present in different plant species that produce benzo[c]phenanthridine alkaloids in culture
?
-
-
-
additional information
Eschscholzia lobbii
the enzyme is specifically present in different plant species that produce benzo[c]phenanthridine alkaloids in culture
?
-
-
-
additional information
Chelidonium majus
the enzyme is specifically present in different plant species that produce benzo[c]phenanthridine alkaloids in culture
?
-
-
-
additional information
Fumaria parviflora
the enzyme is specifically present in different plant species that produce benzo[c]phenanthridine alkaloids in culture
?
-
-
-
additional information
Dicentra cucullaria
the enzyme is specifically present in different plant species that produce benzo[c]phenanthridine alkaloids in culture
?
-
-
-
Organism
Organism
UniProt
Commentary
Textmining
Chelidonium majus
-
-
-
Dicentra cucullaria
-
-
-
Eschscholzia californica
-
strain AST, strain BB, strain ROT
-
Eschscholzia lobbii
-
-
-
Fumaria parviflora
-
-
-
no activity in Berberis stolonifera
-
elicited and non-elicited cells
-
no activity in Catharanthus roseus
-
elicited and non-elicited cells
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell suspension culture
weak activity in non-elicited cells, higher activity in elicited cells
Chelidonium majus
-
cell suspension culture
weak activity in non-elicited cells, higher activity in elicited cells
Dicentra cucullaria
-
cell suspension culture
weak activity in non-elicited cells, higher activity in elicited cells
Eschscholzia californica
-
cell suspension culture
weak activity in non-elicited cells, higher activity in elicited cells
Eschscholzia lobbii
-
cell suspension culture
weak activity in non-elicited cells, higher activity in elicited cells
Fumaria parviflora
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
additional information
the enzyme is specifically present in different plant species that produce benzo[c]phenanthridine alkaloids in culture
7391
Eschscholzia californica
?
-
-
-
-
additional information
the enzyme is specifically present in different plant species that produce benzo[c]phenanthridine alkaloids in culture
7391
Eschscholzia lobbii
?
-
-
-
-
additional information
the enzyme is specifically present in different plant species that produce benzo[c]phenanthridine alkaloids in culture
7391
Chelidonium majus
?
-
-
-
-
additional information
the enzyme is specifically present in different plant species that produce benzo[c]phenanthridine alkaloids in culture
7391
Fumaria parviflora
?
-
-
-
-
additional information
the enzyme is specifically present in different plant species that produce benzo[c]phenanthridine alkaloids in culture
7391
Dicentra cucullaria
?
-
-
-
-
protopine + [reduced NADPH-hemoprotein reductase] + O2
-
7391
Eschscholzia californica
dihydrosanguinarine + [oxidized NADPH-hemoprotein reductase] + H2O
-
7391
Eschscholzia californica
-
protopine + [reduced NADPH-hemoprotein reductase] + O2
-
7391
Eschscholzia lobbii
dihydrosanguinarine + [oxidized NADPH-hemoprotein reductase] + H2O
-
7391
Eschscholzia lobbii
-
protopine + [reduced NADPH-hemoprotein reductase] + O2
-
7391
Chelidonium majus
dihydrosanguinarine + [oxidized NADPH-hemoprotein reductase] + H2O
-
7391
Chelidonium majus
-
protopine + [reduced NADPH-hemoprotein reductase] + O2
-
7391
Fumaria parviflora
dihydrosanguinarine + [oxidized NADPH-hemoprotein reductase] + H2O
-
7391
Fumaria parviflora
-
protopine + [reduced NADPH-hemoprotein reductase] + O2
-
7391
Dicentra cucullaria
dihydrosanguinarine + [oxidized NADPH-hemoprotein reductase] + H2O
-
7391
Dicentra cucullaria
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
-
Eschscholzia californica
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
10
40
about 50% of maximal actvity at 10°C and 40°C
Eschscholzia californica
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Eschscholzia californica
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.3
8.5
pH 6.3: about 35% of maximal activity, pH 8.5: about 40% of maximal activity
Eschscholzia californica
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
the enzyme is linked to cytochrome P-450
Eschscholzia californica
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
the enzyme is linked to cytochrome P-450
Eschscholzia californica
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
CO
-
Eschscholzia californica
cytochrome c
-
Eschscholzia californica
ketoconazole
-
Eschscholzia californica
Metyrapone
-
Eschscholzia californica
Prochloraz
-
Eschscholzia californica
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.002
-
protopine
-
Eschscholzia californica
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Chelidonium majus
-
-
microsome
-
Dicentra cucullaria
-
-
microsome
membrane-bound
Eschscholzia californica
-
-
microsome
-
Eschscholzia lobbii
-
-
microsome
-
Fumaria parviflora
-
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
additional information
Eschscholzia californica
the enzyme is specifically present in different plant species that produce benzo[c]phenanthridine alkaloids in culture
?
-
-
-
additional information
Eschscholzia lobbii
the enzyme is specifically present in different plant species that produce benzo[c]phenanthridine alkaloids in culture
?
-
-
-
additional information
Chelidonium majus
the enzyme is specifically present in different plant species that produce benzo[c]phenanthridine alkaloids in culture
?
-
-
-
additional information
Fumaria parviflora
the enzyme is specifically present in different plant species that produce benzo[c]phenanthridine alkaloids in culture
?
-
-
-
additional information
Dicentra cucullaria
the enzyme is specifically present in different plant species that produce benzo[c]phenanthridine alkaloids in culture
?
-
-
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell suspension culture
weak activity in non-elicited cells, higher activity in elicited cells
Chelidonium majus
-
cell suspension culture
weak activity in non-elicited cells, higher activity in elicited cells
Dicentra cucullaria
-
cell suspension culture
weak activity in non-elicited cells, higher activity in elicited cells
Eschscholzia californica
-
cell suspension culture
weak activity in non-elicited cells, higher activity in elicited cells
Eschscholzia lobbii
-
cell suspension culture
weak activity in non-elicited cells, higher activity in elicited cells
Fumaria parviflora
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
additional information
the enzyme is specifically present in different plant species that produce benzo[c]phenanthridine alkaloids in culture
7391
Eschscholzia californica
?
-
-
-
-
additional information
the enzyme is specifically present in different plant species that produce benzo[c]phenanthridine alkaloids in culture
7391
Eschscholzia lobbii
?
-
-
-
-
additional information
the enzyme is specifically present in different plant species that produce benzo[c]phenanthridine alkaloids in culture
7391
Chelidonium majus
?
-
-
-
-
additional information
the enzyme is specifically present in different plant species that produce benzo[c]phenanthridine alkaloids in culture
7391
Fumaria parviflora
?
-
-
-
-
additional information
the enzyme is specifically present in different plant species that produce benzo[c]phenanthridine alkaloids in culture
7391
Dicentra cucullaria
?
-
-
-
-
protopine + [reduced NADPH-hemoprotein reductase] + O2
-
7391
Eschscholzia californica
dihydrosanguinarine + [oxidized NADPH-hemoprotein reductase] + H2O
-
7391
Eschscholzia californica
-
protopine + [reduced NADPH-hemoprotein reductase] + O2
-
7391
Eschscholzia lobbii
dihydrosanguinarine + [oxidized NADPH-hemoprotein reductase] + H2O
-
7391
Eschscholzia lobbii
-
protopine + [reduced NADPH-hemoprotein reductase] + O2
-
7391
Chelidonium majus
dihydrosanguinarine + [oxidized NADPH-hemoprotein reductase] + H2O
-
7391
Chelidonium majus
-
protopine + [reduced NADPH-hemoprotein reductase] + O2
-
7391
Fumaria parviflora
dihydrosanguinarine + [oxidized NADPH-hemoprotein reductase] + H2O
-
7391
Fumaria parviflora
-
protopine + [reduced NADPH-hemoprotein reductase] + O2
-
7391
Dicentra cucullaria
dihydrosanguinarine + [oxidized NADPH-hemoprotein reductase] + H2O
-
7391
Dicentra cucullaria
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
-
Eschscholzia californica
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
10
40
about 50% of maximal actvity at 10°C and 40°C
Eschscholzia californica
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Eschscholzia californica
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.3
8.5
pH 6.3: about 35% of maximal activity, pH 8.5: about 40% of maximal activity
Eschscholzia californica
Other publictions for EC 1.14.14.98
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746409
Verma
Improved sanguinarine product ...
Papaver somniferum
Protoplasma
251
1359-1371
2014
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
726878
Beaudoin
Isolation and characterization ...
Papaver somniferum
Biochem. Biophys. Res. Commun.
431
597-603
2013
-
-
-
-
-
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
728446
Takemura
Molecular cloning and characte ...
Eschscholzia californica
Phytochemistry
91
100-108
2013
-
-
1
-
-
-
1
2
1
-
-
-
-
4
-
-
-
-
-
-
-
-
5
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
2
1
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7391
Tanahashi
-
Elicitor induction and charact ...
Chelidonium majus, Dicentra cucullaria, Eschscholzia californica, Eschscholzia lobbii, Fumaria parviflora, no activity in Berberis stolonifera, no activity in Catharanthus roseus
Phytochemistry
29
1113-1122
1990
-
-
-
-
-
-
5
1
5
-
-
5
-
7
-
-
-
-
-
5
-
-
10
-
-
1
1
-
-
1
1
-
1
-
-
-
-
-
-
1
-
-
-
-
5
-
1
5
-
-
5
-
-
-
-
-
5
-
-
10
-
1
1
-
-
1
1
-
-
-
-
-
-
-
-