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Literature summary for 1.14.14.96 extracted from
- Chlorogenic acid biosynthesis: Characterization of a light-induced microsomal 5-O-(4-coumaroyl)-D-quinate/shikimate 3'-hydroxylase from carrot (Daucus carota L.) cell suspension cultures (1987), Arch. Biochem. Biophys., 258, 226-232.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| diethyldithiocarbonate | 2fold activation at 0.2-2 mM | Daucus carota |  |
| KCN | 2fold activation at 1-10 mM | Daucus carota | |
| light exposure | 4fold activation after 20 h irradiation at 350 nm | Daucus carota |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome c | 76% inhibition at 0.1 mM | Daucus carota | |
| diethyldicarbonate | 31% inhibition at 2 mM | Daucus carota | |
| p-chloromercuribenzoate | 32% inhibition at 0.5 mM | Daucus carota | |
| Tetcyclacis | 38% inhibition at 0.01 mM | Daucus carota | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Daucus carota | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| trans-5-O-(4-coumaroyl)-D-quinate + [reduced NADPH-hemoprotein reductase] + O2 | Daucus carota | specific for the trans isomer, final step in the chlorogenic acid pathway, reaction is greatly enhanced by irradiation with blue/uv light | trans-5-O-caffeoyl-D-quinate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| trans-5-O-(4-coumaroyl)shikimate + [reduced NADPH-hemoprotein reductase] + O2 | Daucus carota | 70% of the activity compared to trans-5-O-(4-coumaroyl)-D-quinate, specific for the trans isomer | trans-5-O-caffeoylshikimate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Daucus carota | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Daucus carota | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, stable for several months | Daucus carota |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| trans-5-O-(4-coumaroyl)-D-quinate + [reduced NADPH-hemoprotein reductase] + O2 | specific for the trans isomer, final step in the chlorogenic acid pathway, reaction is greatly enhanced by irradiation with blue/uv light | Daucus carota | trans-5-O-caffeoyl-D-quinate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| trans-5-O-(4-coumaroyl)shikimate + [reduced NADPH-hemoprotein reductase] + O2 | 70% of the activity compared to trans-5-O-(4-coumaroyl)-D-quinate, specific for the trans isomer | Daucus carota | trans-5-O-caffeoylshikimate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
half-life: 10 min | Daucus carota |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Daucus carota |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.3 | - |
half-maximal activity | Daucus carota |
| 8.6 | - |
half-maximal activity | Daucus carota |
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Release 2023.1 (January 2023)
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