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Literature summary for 1.14.14.95 extracted from

  • De Kraker, K.J.; Franssen, M.; Dalm, M.; de, G.A.; Bouwmeester, H.
    Biosynthesis of germacrene A carboxylic acid in chicory roots. Demonstration of a cytochrome P450 (+)-germacrene a hydroxylase and NADP+-dependent sesquiterpenoid dehydrogenase(s) involved in sesquiterpene lactone biosynthesis (2001), Plant Physiol., 125, 1930-1940.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.14.95

Inhibitors

Inhibitors Comment Organism Structure
aminobenzotriazole 26% inhibition at 0.1 mM Cichorium intybus
carbon monoxide blue-light reversible inhibition, an atmosphere of 80% CO plus 20% O2 inhibits (+)-germacrene A hydroxylase by 69% Cichorium intybus
clotrimazole 016% inhibition at 0.1 mM Cichorium intybus
cytochrome c 97% inhibition at 0.1 mM Cichorium intybus
Metyrapone 23% inhibition at 1 mM Cichorium intybus
miconazole 30% inhibition at 0.1 mM Cichorium intybus
additional information flushing the reaction mixture for 1.5 min with argon prior to incubation causes a 69% decrease of enzyme activity because of O2 depletion Cichorium intybus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Cichorium intybus
-
-

Organism

Organism UniProt Comment Textmining
Cichorium intybus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
root
-
 Cichorium intybus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-germacrene A + [reduced NADPH-hemoprotein reductase] + O2 100% activity Cichorium intybus germacra-1(10),4,11(13)-trien-12-ol + [oxidized NADPH-hemoprotein reductase] + H2O germacra-1(10),4,11(13)-trien-12-ol is also named costol ?
(+)-germacrene A + [reduced NADPH-hemoprotein reductase] + O2 30% activity with NADH as compared to NADPH Cichorium intybus germacra-1(10),4,11(13)-trien-12-ol + [oxidized NADPH-hemoprotein reductase] + H2O germacra-1(10),4,11(13)-trien-12-ol is also named costol ?
beta-elemene + [reduced NADPH-hemoprotein reductase] + O2 the amount of (+)-beta-elemene hydroxylated is 2times less than that of (-)-beta-elemene Cichorium intybus elema-1,3,11(13)-trien-12-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
germacra-1(10),4,11(13)-trien-12-ol + 2 O2 + 2 [reduced NADPH-hemoprotein reductase]
-
 Cichorium intybus germacra-1(10),4,11(13)-trien-12-oate + 2 [oxidized NADPH-hemoprotein reductase] + 3 H2O
-
 ?

Synonyms

Synonyms Comment Organism
(+)-germacrene A hydroxylase
-
 Cichorium intybus
NADP+-dependent sesquiterpenoid dehydrogenase
-
 Cichorium intybus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Cichorium intybus

pH Range

pH Minimum pH Maximum Comment Organism
6 9 60% of maximal enzyme activity at pH 7.5 and 9.0 (no difference in activity between bis-Tris and Tris buffer) Cichorium intybus

Cofactor

Cofactor Comment Organism Structure
cytochrome P-450 required for activity, the combination of NADPH with NADH shows an additive effect on enzyme activity (120%) Cichorium intybus
flavin an assay buffer without flavins (FAD and FMN) gives 18% loss of hydroxylase activity. However, omitting these flavins from the extraction buffer results in a loss of more than 70% in enzyme activity Cichorium intybus
NADP+ dependent on Cichorium intybus