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Literature summary for 1.14.14.93 extracted from
- Induction of phytoalexin synthesis in soybean. Stereospecific 3,9-dihydroxypterocarpan 6a-hydroxylase from elicitor-induced soybean cell cultures (1984), Eur. J. Biochem., 142, 127-131.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome c | - |
Glycine max |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Glycine max | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | increases activity | Glycine max | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (6aR,11aR)-3,9-dihydroxypterocarpan + [reduced NADPH-hemoprotein reductase] + O2 | Glycine max | the product of the reaction is the biosynthetic precursor of the phytoalexin glyceollin in soybean | (6aS,11aS)-3,6a,9-trihydroxypterocarpan + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Glycine max | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Glycine max | - |
| seedling | - |
Glycine max | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, storage of microsomes, 50% loss of activity in 3-4 weeks | Glycine max |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (6aR,11aR)-3,9-dihydroxypterocarpan + [reduced NADPH-hemoprotein reductase] + O2 | - |
Glycine max | (6aS,11aS)-3,6a,9-trihydroxypterocarpan + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| (6aR,11aR)-3,9-dihydroxypterocarpan + [reduced NADPH-hemoprotein reductase] + O2 | the product of the reaction is the biosynthetic precursor of the phytoalexin glyceollin in soybean | Glycine max | (6aS,11aS)-3,6a,9-trihydroxypterocarpan + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Glycine max |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
75% of activity maximum at 30°C | Glycine max |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
half-life: 9 min, in 0.08 M potassium phosphate buffer, pH 7.5, 14 mM 2-mercaptoethanol, 20% sucrose, crude enzyme extract | Glycine max |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | 8.2 | in Tris/HCl and potassium phosphate | Glycine max |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome P450 | possibly a heme-thiolate protein | Glycine max | |
| FAD | at 0.05 mM with 0.05 mM FMN and 0.01 mM NADPH: 132% relative activity to NADPH alone | Glycine max | |
| FMN | at 0.05 mM with 0.05 mM FAD and 0.01 mM NADPH: 132% relative activity to NADPH alone | Glycine max | |
| additional information | with NADH, ascorbic acid, FAD, FMN or 6,7-dimethyl-5,6,7,8-tetrahydropterine alone no activity can be detected | Glycine max |
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