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Literature summary for 1.14.14.92 extracted from
- Molecular characterization of cytochrome P450 catalyzing hydroxylation of benzoates from the white-rot fungus Phanerochaete chrysosporium (2005), Biochem. Biophys. Res. Commun., 334, 1184-1190.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | benzoic acid induces transcription of the gene encoding the enzyme and has a regulatory effect | Phanerodontia chrysosporium |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene CYP1f, DNA and amino acid sequence determination and analysis, five introns and six exons, functional expression in Pichia pastoris | Phanerodontia chrysosporium |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Phanerodontia chrysosporium | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | heme iron, the heme-binding domain FSFGPRSCVG at residues 421430 is located in the C-terminal region | Phanerodontia chrysosporium |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
x * 60000, about, amino acid sequence calculation | Phanerodontia chrysosporium |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzoic acid + [reduced NADPH-hemoprotein reductase] + O2 | Phanerodontia chrysosporium | benzoic acid has a regulatory effect on the enzyme | 4-hydroxybenzoic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phanerodontia chrysosporium | Q4H2B4 | white-rot fungus, a lignin-degrading basidiomycete, ATCC 34541 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-methylbenzoic acid + NADPH + O2 | - |
Phanerodontia chrysosporium | 4-hydroxy-2-methylbenzoic acid + NADP+ + H2O | - |
? | |
| 3-hydroxybenzoic acid + [reduced NADPH-hemoprotein reductase] + O2 | reaction of 3-hydroxybenzoate 4-monooxygenase, EC 1.14.13.23 | Phanerodontia chrysosporium | 3,4-dihydroxybenzoic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 3-methylbenzoic acid + [reduced NADPH-hemoprotein reductase] + O2 | - |
Phanerodontia chrysosporium | 4-hydroxy-3-methylbenzoic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| benzoic acid + [reduced NADPH-hemoprotein reductase] + O2 | - |
Phanerodontia chrysosporium | 4-hydroxybenzoic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| benzoic acid + [reduced NADPH-hemoprotein reductase] + O2 | benzoic acid has a regulatory effect on the enzyme | Phanerodontia chrysosporium | 4-hydroxybenzoic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 60000, about, amino acid sequence calculation | Phanerodontia chrysosporium |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Cyp1 | - |
Phanerodontia chrysosporium |
| More | the enzyme belongs to the cytochrome P450 CYP53 family, cf. EC 1.14.13.23 | Phanerodontia chrysosporium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Phanerodontia chrysosporium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Phanerodontia chrysosporium |
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Release 2023.1 (January 2023)
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