BRENDA - Enzyme Database show
show all sequences of 1.14.14.92

Purification, properties and induction of a specific benzoate-4-hydroxylase from Aspergillus niger (UBC 814)

Reddy, C.C.; Vaidyanathan, C.S.; Biochim. Biophys. Acta 384, 46-57 (1975)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
ascorbic acid
30% of activity with NADPH as electron donor
Aspergillus niger
General Stability
General Stability
Organism
benzoate stabilizes
Aspergillus niger
EDTA stabilizes
Aspergillus niger
glutathione stabilizes
Aspergillus niger
Inhibitors
Inhibitors
Commentary
Organism
Structure
2,2'-dipyridyl
0.05 mM; 50% inhibition
Aspergillus niger
8-hydroxyquinoline
5 mM, complete inhibition
Aspergillus niger
Cu2+
0.001-0.01 mM, complete inhibition
Aspergillus niger
diethyldithiocarbamate
5 mM, 95% inhibition
Aspergillus niger
Hg2+
0.001-0.01 mM, complete inhibition
Aspergillus niger
Mg2+
0.001-0.01 mM, complete inhibition
Aspergillus niger
Mn2+
0.001-0.01 mM, complete inhibition
Aspergillus niger
Mo2+
0.001-0.01 mM, complete inhibition
Aspergillus niger
N-ethylmaleimide
-
Aspergillus niger
o-phenanthroline
0.05 mM, 70% inhibition
Aspergillus niger
oxalate
2 mM, 20% inhibition
Aspergillus niger
p-chloromercuribenzoate
-
Aspergillus niger
Zn2+
0.001-0.01 mM, complete inhibition
Aspergillus niger
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.13
-
Benzoate
-
Aspergillus niger
0.16
-
NADPH
-
Aspergillus niger
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
required for activity
Aspergillus niger
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Aspergillus niger
-
-
-
Aspergillus niger UBC 814
-
-
-
Purification (Commentary)
Commentary
Organism
protamine sulfate, tricalcium phosphate gel, DEAE-cellulose, alumina C-gamma gel
Aspergillus niger
Source Tissue
Source Tissue
Commentary
Organism
Textmining
mycelium
-
Aspergillus niger
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0888
-
-
Aspergillus niger
Storage Stability
Storage Stability
Organism
4°C, 24 h, 40% loss of activity
Aspergillus niger
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
benzoate + [reduced NADPH-hemoprotein reductase] + O2
specific for benzoate, NADPH is absolutely required, 30% activity with ascorbate as electron donor
440256
Aspergillus niger
4-hydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
440256
Aspergillus niger
?
benzoate + [reduced NADPH-hemoprotein reductase] + O2
specific for benzoate, NADPH is absolutely required, 30% activity with ascorbate as electron donor
440256
Aspergillus niger UBC 814
4-hydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
440256
Aspergillus niger UBC 814
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
-
Aspergillus niger
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
25
38
rapid decrease of activity above 38°C and below 25°C
Aspergillus niger
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.2
-
-
Aspergillus niger
pH Range
pH Minimum
pH Maximum
Commentary
Organism
4.5
8
approx. 35% of maximal activity at pH 4.5, approx. 15% of maximal activity at pH 8.0
Aspergillus niger
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
5.5
7.5
-
Aspergillus niger
Cofactor
Cofactor
Commentary
Organism
Structure
tetrahydropteridine
required as prosthetic group, maximal activity with 6,7-dimethyl 5,6,7,8-tetrahydropteridine, 60% activity with biopterine and tetrahydrofolic acid
Aspergillus niger
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
ascorbic acid
30% of activity with NADPH as electron donor
Aspergillus niger
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
tetrahydropteridine
required as prosthetic group, maximal activity with 6,7-dimethyl 5,6,7,8-tetrahydropteridine, 60% activity with biopterine and tetrahydrofolic acid
Aspergillus niger
General Stability (protein specific)
General Stability
Organism
benzoate stabilizes
Aspergillus niger
EDTA stabilizes
Aspergillus niger
glutathione stabilizes
Aspergillus niger
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2,2'-dipyridyl
0.05 mM; 50% inhibition
Aspergillus niger
8-hydroxyquinoline
5 mM, complete inhibition
Aspergillus niger
Cu2+
0.001-0.01 mM, complete inhibition
Aspergillus niger
diethyldithiocarbamate
5 mM, 95% inhibition
Aspergillus niger
Hg2+
0.001-0.01 mM, complete inhibition
Aspergillus niger
Mg2+
0.001-0.01 mM, complete inhibition
Aspergillus niger
Mn2+
0.001-0.01 mM, complete inhibition
Aspergillus niger
Mo2+
0.001-0.01 mM, complete inhibition
Aspergillus niger
N-ethylmaleimide
-
Aspergillus niger
o-phenanthroline
0.05 mM, 70% inhibition
Aspergillus niger
oxalate
2 mM, 20% inhibition
Aspergillus niger
p-chloromercuribenzoate
-
Aspergillus niger
Zn2+
0.001-0.01 mM, complete inhibition
Aspergillus niger
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.13
-
Benzoate
-
Aspergillus niger
0.16
-
NADPH
-
Aspergillus niger
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
required for activity
Aspergillus niger
Purification (Commentary) (protein specific)
Commentary
Organism
protamine sulfate, tricalcium phosphate gel, DEAE-cellulose, alumina C-gamma gel
Aspergillus niger
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
mycelium
-
Aspergillus niger
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0888
-
-
Aspergillus niger
Storage Stability (protein specific)
Storage Stability
Organism
4°C, 24 h, 40% loss of activity
Aspergillus niger
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
benzoate + [reduced NADPH-hemoprotein reductase] + O2
specific for benzoate, NADPH is absolutely required, 30% activity with ascorbate as electron donor
440256
Aspergillus niger
4-hydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
440256
Aspergillus niger
?
benzoate + [reduced NADPH-hemoprotein reductase] + O2
specific for benzoate, NADPH is absolutely required, 30% activity with ascorbate as electron donor
440256
Aspergillus niger UBC 814
4-hydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
440256
Aspergillus niger UBC 814
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
-
Aspergillus niger
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
25
38
rapid decrease of activity above 38°C and below 25°C
Aspergillus niger
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.2
-
-
Aspergillus niger
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
4.5
8
approx. 35% of maximal activity at pH 4.5, approx. 15% of maximal activity at pH 8.0
Aspergillus niger
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
5.5
7.5
-
Aspergillus niger
Other publictions for EC 1.14.14.92
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741716
Antunes
A benzoate-activated promoter ...
Aspergillus niger
Appl. Microbiol. Biotechnol.
100
5479-5489
2016
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1
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1
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1
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742764
Korosec
Antifungal activity of cinnam ...
Curvularia lunata
J. Appl. Microbiol.
116
955-966
2014
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2
1
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1
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5
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1
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2
1
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1
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1
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-
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2
2
-
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-
725603
Berne
Virtual screening yields inhib ...
Curvularia lunata
J. Chem. Inf. Model.
52
3053-3063
2012
-
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-
1
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2
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2
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686803
Kim
Molecular cloning and function ...
Chromohalobacter sp. HS2
FEMS Microbiol. Lett.
280
235-241
2008
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1
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1
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1
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3
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1
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671533
Shiningavamwe
Heterologous expression of the ...
Cystobasidium minutum, Cystobasidium minutum CBS 2177
Appl. Microbiol. Biotechnol.
72
323-329
2006
-
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1
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2
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6
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1
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2
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1
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2
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1
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2
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671704
Matsuzaki
Molecular characterization of ...
Phanerochaete chrysosporium
Biochem. Biophys. Res. Commun.
334
1184-1190
2005
1
-
1
-
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1
1
1
1
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1
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5
1
1
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1
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1
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1
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1
1
1
1
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5
1
1
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1
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440261
Faber
Purification and characterizat ...
Aspergillus niger
Arch. Biochem. Biophys.
394
245-254
2001
-
-
-
-
-
-
-
9
-
-
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-
-
1
-
-
1
-
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-
12
-
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-
13
-
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-
9
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1
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-
12
-
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13
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-
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440259
Fukuda
Cytochrome P450rm from Rhodoto ...
Cystobasidium minutum
J. Biochem.
119
314-318
1996
-
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3
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1
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4
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2
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3
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1
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1
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3
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1
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2
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3
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440260
Van Den Brink
Optimization of the benzoate-i ...
Aspergillus niger, Aspergillus niger N204
Appl. Microbiol. Biotechnol.
46
360-364
1996
-
-
1
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2
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2
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1
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1
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2
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1
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-
-
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-
-
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440254
McNamee
Properties of a membrane-assoc ...
Rhodotorula graminis
Biochem. Biophys. Res. Commun.
129
485-492
1985
-
-
1
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15
2
1
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3
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1
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1
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1
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15
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2
1
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1
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1
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4804
Durham
Initial reactions involved in ...
Rhodotorula graminis
J. Bacteriol.
160
778-780
1984
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1
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440258
Shailubhai
-
Degradation of chlorinated der ...
Aspergillus niger
FEMS Microbiol. Lett.
18
279-282
1983
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-
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1
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1
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1
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1
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1
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1
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440255
Reddy
Purification and properties of ...
Pseudomonas sp.
Arch. Biochem. Biophys.
177
488-498
1976
3
-
-
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2
9
3
-
1
1
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1
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1
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1
2
3
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1
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1
1
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1
1
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3
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1
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2
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9
1
3
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1
1
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1
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1
2
3
-
1
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1
1
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-
-
-
-
-
-
440256
Reddy
Purification, properties and i ...
Aspergillus niger, Aspergillus niger UBC 814
Biochim. Biophys. Acta
384
46-57
1975
1
-
-
-
-
3
13
2
-
1
-
-
-
6
-
-
1
-
-
2
1
1
2
-
1
1
-
-
1
1
1
1
-
-
-
1
-
-
1
-
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3
-
13
-
2
-
1
-
-
-
-
-
1
-
2
1
1
2
-
1
1
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1
1
1
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-
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-
-