Any feedback?
Literature summary for 1.14.14.92 extracted from
- Properties of a membrane-associated benzoate-4-hydroxylase from Rhodotorula graminis (1985), Biochem. Biophys. Res. Commun., 129, 485-492.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Rhodotorula graminis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| aminopterin | 0.5 mM, 10% inhibition | Rhodotorula graminis |  |
| Benzyl acetate | 0.5 mM, 21% inhibition | Rhodotorula graminis | |
| benzyl alcohol | 0.5 mM, 10% inhibition | Rhodotorula graminis | |
| benzylformate | 0.5 mM, 10% inhibition | Rhodotorula graminis | |
| CO | 44% inhibition | Rhodotorula graminis | |
| diethyldithiocarbamate | 0.5 mM, 21% inhibition | Rhodotorula graminis | |
| iodoacetamide | 5 mM, 43% inhibition | Rhodotorula graminis | |
| iodoacetate | 5 mM, 23% inhibition | Rhodotorula graminis | |
| KCN | 30 mM, 21% inhibition | Rhodotorula graminis | |
| p-hydroxymercuribenzoate | 0.052 mM, 39% inhibition | Rhodotorula graminis | |
| phenylalanine | 0.5 mM, 21% inhibition | Rhodotorula graminis | |
| Quinacrine | 0.5 mM, 47% inhibition | Rhodotorula graminis | |
| quinine-HCl | 0.5 mM, 42% inhibition | Rhodotorula graminis | |
| SKF-525A | cytochorme P450 inhibitor, 1 mM, 51% inhibition | Rhodotorula graminis | |
| trans-cinnamate | 0.5 mM, 55% inhibition | Rhodotorula graminis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.019 | - |
NADPH | - |
Rhodotorula graminis | |
| 0.029 | - |
benzoate | - |
Rhodotorula graminis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | membrane associated | Rhodotorula graminis | 16020 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodotorula graminis | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzoate + NADPH + H+ + O2 | - |
Rhodotorula graminis | 4-hydroxybenzoate + NADP+ + H2O | - |
r | |
| benzoate + [reduced NADPH-hemoprotein reductase] + O2 | - |
Rhodotorula graminis | 4-hydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
- |
Rhodotorula graminis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | 2fold stimulation of NADPH oxidation rate | Rhodotorula graminis | |
| additional information | pteridine independent activity | Rhodotorula graminis |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
