BRENDA - Enzyme Database
show all sequences of 1.14.14.92

Properties of a membrane-associated benzoate-4-hydroxylase from Rhodotorula graminis

McNamee, C.; Durham, D.R.; Biochem. Biophys. Res. Commun. 129, 485-492 (1985)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
-
Rhodotorula graminis
Inhibitors
Inhibitors
Commentary
Organism
Structure
aminopterin
0.5 mM, 10% inhibition
Rhodotorula graminis
Benzyl acetate
0.5 mM, 21% inhibition
Rhodotorula graminis
benzyl alcohol
0.5 mM, 10% inhibition
Rhodotorula graminis
benzylformate
0.5 mM, 10% inhibition
Rhodotorula graminis
CO
44% inhibition
Rhodotorula graminis
diethyldithiocarbamate
0.5 mM, 21% inhibition
Rhodotorula graminis
iodoacetamide
5 mM, 43% inhibition
Rhodotorula graminis
iodoacetate
5 mM, 23% inhibition
Rhodotorula graminis
KCN
30 mM, 21% inhibition
Rhodotorula graminis
p-hydroxymercuribenzoate
0.052 mM, 39% inhibition
Rhodotorula graminis
phenylalanine
0.5 mM, 21% inhibition
Rhodotorula graminis
Quinacrine
0.5 mM, 47% inhibition
Rhodotorula graminis
quinine-HCl
0.5 mM, 42% inhibition
Rhodotorula graminis
SKF-525A
cytochorme P450 inhibitor, 1 mM, 51% inhibition
Rhodotorula graminis
trans-cinnamate
0.5 mM, 55% inhibition
Rhodotorula graminis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.019
-
NADPH
-
Rhodotorula graminis
0.029
-
benzoate
-
Rhodotorula graminis
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
membrane associated
Rhodotorula graminis
16020
-
Organism
Organism
UniProt
Commentary
Textmining
Rhodotorula graminis
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
benzoate + [reduced NADPH-hemoprotein reductase] + O2
-
440254
Rhodotorula graminis
4-hydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
440254
Rhodotorula graminis
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.6
-
-
Rhodotorula graminis
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
2fold stimulation of NADPH oxidation rate
Rhodotorula graminis
additional information
pteridine independent activity
Rhodotorula graminis
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Rhodotorula graminis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
2fold stimulation of NADPH oxidation rate
Rhodotorula graminis
additional information
pteridine independent activity
Rhodotorula graminis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
aminopterin
0.5 mM, 10% inhibition
Rhodotorula graminis
Benzyl acetate
0.5 mM, 21% inhibition
Rhodotorula graminis
benzyl alcohol
0.5 mM, 10% inhibition
Rhodotorula graminis
benzylformate
0.5 mM, 10% inhibition
Rhodotorula graminis
CO
44% inhibition
Rhodotorula graminis
diethyldithiocarbamate
0.5 mM, 21% inhibition
Rhodotorula graminis
iodoacetamide
5 mM, 43% inhibition
Rhodotorula graminis
iodoacetate
5 mM, 23% inhibition
Rhodotorula graminis
KCN
30 mM, 21% inhibition
Rhodotorula graminis
p-hydroxymercuribenzoate
0.052 mM, 39% inhibition
Rhodotorula graminis
phenylalanine
0.5 mM, 21% inhibition
Rhodotorula graminis
Quinacrine
0.5 mM, 47% inhibition
Rhodotorula graminis
quinine-HCl
0.5 mM, 42% inhibition
Rhodotorula graminis
SKF-525A
cytochorme P450 inhibitor, 1 mM, 51% inhibition
Rhodotorula graminis
trans-cinnamate
0.5 mM, 55% inhibition
Rhodotorula graminis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.019
-
NADPH
-
Rhodotorula graminis
0.029
-
benzoate
-
Rhodotorula graminis
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
membrane associated
Rhodotorula graminis
16020
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
benzoate + [reduced NADPH-hemoprotein reductase] + O2
-
440254
Rhodotorula graminis
4-hydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
440254
Rhodotorula graminis
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.6
-
-
Rhodotorula graminis
Other publictions for EC 1.14.14.92
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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1
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2
2
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725603
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Virtual screening yields inhib ...
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686803
Kim
Molecular cloning and function ...
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FEMS Microbiol. Lett.
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1
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3
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1
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671533
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Appl. Microbiol. Biotechnol.
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6
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1
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2
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1
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671704
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334
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1
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1
1
1
1
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1
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5
1
2
1
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1
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1
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1
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1
1
1
1
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5
1
1
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1
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440261
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Purification and characterizat ...
Aspergillus niger
Arch. Biochem. Biophys.
394
245-254
2001
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9
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1
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1
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12
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13
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9
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1
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12
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13
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440259
Fukuda
Cytochrome P450rm from Rhodoto ...
Cystobasidium minutum
J. Biochem.
119
314-318
1996
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3
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1
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4
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3
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440260
Van Den Brink
Optimization of the benzoate-i ...
Aspergillus niger, Aspergillus niger N204
Appl. Microbiol. Biotechnol.
46
360-364
1996
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1
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-
-
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2
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2
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1
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-
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-
-
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-
440254
McNamee
Properties of a membrane-assoc ...
Rhodotorula graminis
Biochem. Biophys. Res. Commun.
129
485-492
1985
-
-
1
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-
15
2
1
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3
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1
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1
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1
2
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15
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2
1
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1
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1
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4804
Durham
Initial reactions involved in ...
Rhodotorula graminis
J. Bacteriol.
160
778-780
1984
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440258
Shailubhai
-
Degradation of chlorinated der ...
Aspergillus niger
FEMS Microbiol. Lett.
18
279-282
1983
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1
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440255
Reddy
Purification and properties of ...
Pseudomonas sp.
Arch. Biochem. Biophys.
177
488-498
1976
3
-
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2
9
3
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1
1
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1
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1
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1
2
3
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1
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1
1
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1
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3
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9
1
3
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1
1
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1
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1
2
3
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1
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1
1
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440256
Reddy
Purification, properties and i ...
Aspergillus niger, Aspergillus niger UBC 814
Biochim. Biophys. Acta
384
46-57
1975
1
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3
13
2
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1
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6
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1
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2
1
1
2
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1
1
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1
1
1
1
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1
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3
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13
-
2
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1
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1
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1
1
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1
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1
1
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