Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.14.91 extracted from

  • Chen, H.C.; Li, Q.; Shuford, C.M.; Liu, J.; Muddiman, D.C.; Sederoff, R.R.; Chiang, V.L.
    Membrane protein complexes catalyze both 4- and 3-hydroxylation of cinnamic acid derivatives in monolignol biosynthesis (2011), Proc. Natl. Acad. Sci. USA, 108, 21253-21258.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0036
-
trans-cinnamate isoforms C4H1/C4H2/p-coumaroyl ester 3-hydroxylase C3H3 complex, pH 7.0, 20°C Populus trichocarpa
0.0037
-
trans-cinnamate isoforms C4H1/C4H2 complex, pH 7.0, 20°C Populus trichocarpa
0.0048
-
trans-cinnamate isoform C4H1/p-coumaroyl ester 3-hydroxylase C3H3 complex, pH 7.0, 20°C Populus trichocarpa
0.0059
-
trans-cinnamate isoform C4H2/p-coumaroyl ester 3-hydroxylase C3H3 complex, pH 7.0, 20°C Populus trichocarpa
0.007
-
trans-cinnamate isoform C4H1, pH 7.0, 20°C Populus trichocarpa
0.04
-
trans-cinnamate isoform C4H2, pH 7.0, 20°C Populus trichocarpa

Organism

Organism UniProt Comment Textmining
Populus trichocarpa
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
trans-cinnamate + [reduced NADPH-hemoprotein reductase] + O2
-
Populus trichocarpa 4-hydroxycinnamate + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

General Information

General Information Comment Organism
physiological function cinnamic acid 4-hydroxylases isoforms C4H1 and C4H2 and a p-coumaroyl ester 3-hydroxylase C3H3 interact, forming heterodimeric C4H1/C4H2, C4H1/C3H3, and C4H2/C3H3 and heterotrimeric PtrC4H1/C4H2/C3H3 membrane protein complexes. Enzyme kinetics demonstrates that the enzymatic efficiency for any of the complexes is 70-6500 times greater than that of the individual proteins Populus trichocarpa