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Literature summary for 1.14.14.91 extracted from

  • Chen, H.; Jiang, H.; Morgan, J.A.
    Non-natural cinnamic acid derivatives as substrates of cinnamate 4-hydroxylase (2007), Phytochemistry, 68, 306-311.
    View publication on PubMed
Search for more literature for 1.14.14.91

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae strain WAT11 Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0005
-
 trans-cinnamate 30°C Arabidopsis thaliana
0.0005
-
 trans-cinnamate 0.0005-0.04 mM substrate and 1 mM NADPH, at 30°C Arabidopsis thaliana
0.0022
-
 2-chlorocinnamic acid 0.0005-0.04 mM substrate and 1 mM NADPH, at 30°C Arabidopsis thaliana
0.0023
-
 2-fluorocinnamic acid 0.0005-0.04 mM substrate and 1 mM NADPH, at 30°C Arabidopsis thaliana
0.0131
-
 3-hydroxycinnamic acid 0.0005-0.04 mM substrate and 1 mM NADPH, at 30°C Arabidopsis thaliana
0.0131
-
 (2E)-3-(2-nitrophenyl)prop-2-enoic acid 0.0005-0.04 mM substrate and 1 mM NADPH, at 30°C Arabidopsis thaliana
0.0131
-
 (2E)-3-(2-methylphenyl)prop-2-enoic acid 0.0005-0.04 mM substrate and 1 mM NADPH, at 30°C Arabidopsis thaliana
0.0131
-
 (2E)-3-(2-methoxyphenyl)prop-2-enoic acid 0.0005-0.04 mM substrate and 1 mM NADPH, at 30°C Arabidopsis thaliana
0.0131
-
 (2E)-3-(2-ethoxyphenyl)prop-2-enoic acid 0.0005-0.04 mM substrate and 1 mM NADPH, at 30°C Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-
Arabidopsis thaliana
-
 recombinant
-

Purification (Commentary)

Purification (Comment) Organism
-
 Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E)-3-(2,3-dimethoxyphenyl)prop-2-enoic acid + [reduced NADPH-hemoprotein reductase] + O2
-
 Arabidopsis thaliana (2E)-3-(4-hydroxy-2,3-dimethoxyphenyl)prop-2-enoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
(2E)-3-(2-chlorophenyl)prop-2-enoic acid + [reduced NADPH-hemoprotein reductase] + O2
-
 Arabidopsis thaliana (2E)-3-(2-chloro-4-hydroxyphenyl)prop-2-enoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
(2E)-3-(2-ethoxyphenyl)prop-2-enoic acid + [reduced NADPH-hemoprotein reductase] + O2
-
 Arabidopsis thaliana (2E)-3-(2-ethoxy-4-hydroxyphenyl)prop-2-enoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
(2E)-3-(2-fluorophenyl)prop-2-enoic acid + [reduced NADPH-hemoprotein reductase] + O2
-
 Arabidopsis thaliana (2E)-3-(2-fluoro-4-hydroxyphenyl)prop-2-enoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
(2E)-3-(2-hydroxyphenyl)prop-2-enoic acid + [reduced NADPH-hemoprotein reductase] + O2
-
 Arabidopsis thaliana (2E)-3-(2,4-dihydroxyphenyl)prop-2-enoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
(2E)-3-(2-methoxyphenyl)prop-2-enoic acid + [reduced NADPH-hemoprotein reductase] + O2
-
 Arabidopsis thaliana (2E)-3-(4-hydroxy-2-methoxyphenyl)prop-2-enoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
(2E)-3-(2-methylphenyl)prop-2-enoic acid + [reduced NADPH-hemoprotein reductase] + O2
-
 Arabidopsis thaliana (2E)-3-(4-hydroxy-2-methylphenyl)prop-2-enoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
(2E)-3-(2-nitrophenyl)prop-2-enoic acid + [reduced NADPH-hemoprotein reductase] + O2
-
 Arabidopsis thaliana (2E)-3-(4-hydroxy-2-nitrophenyl)prop-2-enoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
(2E)-3-(3-chlorophenyl)prop-2-enoic acid + [reduced NADPH-hemoprotein reductase] + O2
-
 Arabidopsis thaliana (2E)-3-(3-chloro-4-hydroxyphenyl)prop-2-enoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
(2E)-3-(3-fluorophenyl)prop-2-enoic acid + [reduced NADPH-hemoprotein reductase] + O2
-
 Arabidopsis thaliana (2E)-3-(3-fluoro-4-hydroxyphenyl)prop-2-enoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
(2E)-3-(3-hydroxyphenyl)prop-2-enoic acid + [reduced NADPH-hemoprotein reductase] + O2
-
 Arabidopsis thaliana (2E)-3-(3,4-dihydroxyphenyl)prop-2-enoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
(2E)-3-(3-methoxyphenyl)prop-2-enoic acid + [reduced NADPH-hemoprotein reductase] + O2
-
 Arabidopsis thaliana (2E)-3-(4-hydroxy-3-methoxyphenyl)prop-2-enoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
(2E)-3-(3-methylphenyl)prop-2-enoic acid + [reduced NADPH-hemoprotein reductase] + O2
-
 Arabidopsis thaliana (2E)-3-(4-hydroxy-3-methylphenyl)prop-2-enoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
(2E)-3-(3-nitrophenyl)prop-2-enoic acid + [reduced NADPH-hemoprotein reductase] + O2
-
 Arabidopsis thaliana (2E)-3-(4-hydroxy-3-nitrophenyl)prop-2-enoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
2-chlorocinnamic acid + [reduced NADPH-hemoprotein reductase] + O2
-
 Arabidopsis thaliana ? + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
2-fluorocinnamic acid + [reduced NADPH-hemoprotein reductase] + O2
-
 Arabidopsis thaliana ? + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
3-hydroxycinnamic acid + [reduced NADPH-hemoprotein reductase] + O2
-
 Arabidopsis thaliana ? + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
trans-cinnamate + [reduced NADPH-hemoprotein reductase] + O2
-
 Arabidopsis thaliana 4-hydroxycinnamate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Synonyms

Synonyms Comment Organism
C4H
-
 Arabidopsis thaliana
cinnamate 4-hydroxylase
-
 Arabidopsis thaliana
CYP73A5
-
 Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.72
-
 trans-cinnamate 30°C Arabidopsis thaliana