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Literature summary for 1.14.14.91 extracted from
- Cinnamic acid 4-hydroxylase from cell cultures of the hornwort Anthoceros agrestis (2003), Planta, 217, 96-101.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome c | hydroxylase activity with NADPH is strongly inhibited, activity with NADH is less susceptible | Anthoceros agrestis |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.005 | - |
trans-cinnamate | - |
Anthoceros agrestis | |
| 0.06 | - |
NADPH | - |
Anthoceros agrestis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| trans-cinnamate + [reduced NADPH-hemoprotein reductase] + O2 | Anthoceros agrestis | enzyme is involved in the biosynthesis of rosmarinic acid | 4-hydroxycinnamate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Anthoceros agrestis | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Anthoceros agrestis | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| trans-cinnamate + [reduced NADPH-hemoprotein reductase] + O2 | - |
Anthoceros agrestis | 4-hydroxycinnamate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| trans-cinnamate + [reduced NADPH-hemoprotein reductase] + O2 | enzyme is involved in the biosynthesis of rosmarinic acid | Anthoceros agrestis | 4-hydroxycinnamate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| trans-cinnamate + [reduced NADPH-hemoprotein reductase] + O2 | reaction with NADH shows 50% of the activity with NADPH | Anthoceros agrestis | 4-hydroxycinnamate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cinnamic acid 4-hydroxylase | - |
Anthoceros agrestis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
- |
Anthoceros agrestis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
40 | 0°C: 45% of maximal activity, 40°C: 60% of maximal activity | Anthoceros agrestis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Anthoceros agrestis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.3 | 8.2 | 50% of maximal activity at pH 6.3 and pH 8.2 | Anthoceros agrestis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome P450 | - |
Anthoceros agrestis | |
| NADH | 50% of the activity with NADPH | Anthoceros agrestis | |
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Release 2023.1 (January 2023)
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