Literature summary for 1.14.14.91 extracted from

Pierrel, M.A.; Batard, Y.; Kazmaier, M.; Mignotte-Vieux, C.; Durst, F.; Werck-Reichhart, D.
Catalytic properties of the plant cytochrome P450 CYP73 expressed in yeast. Substrate specificity of a cinnamate hydroxylase (1994), Eur. J. Biochem., 224, 835-844.

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Inhibitors

Inhibitors	Comment	Organism	Structure
1-aminobenzotriazole	-	Helianthus tuberosus
2,4-dichloro-1-prop-2-ynyloxy-benzene	-	Helianthus tuberosus
2-isopropyl-4-pentenamide	-	Helianthus tuberosus
phenoxy-1-propyne	-	Helianthus tuberosus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.005	-	trans-cinnamate	-	Helianthus tuberosus
0.555	-	7-ethoxycoumarin	-	Helianthus tuberosus
1.919	-	p-chloro-N-methylaniline	-	Helianthus tuberosus
4	-	7-methoxycoumarin	-	Helianthus tuberosus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
microsome	-	Helianthus tuberosus	-	-

Organism

Organism	UniProt	Comment	Textmining
Helianthus tuberosus	-	expressed by an optimized yeast system	-

Source Tissue

Source Tissue	Comment	Organism	Textmining

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
7-ethoxycoumarin + [reduced NADPH-hemoprotein reductase] + O2	demethylase	Helianthus tuberosus	?	-	?
7-methoxycoumarin + [reduced NADPH-hemoprotein reductase] + O2	demethylase	Helianthus tuberosus	umbelliferone + ? + [oxidized NADPH-hemoprotein reductase] + H2O	-	?
chlorotoluron + [reduced NADPH-hemoprotein reductase] + O2	methylhydroxylase activity	Helianthus tuberosus	?	-	?
p-chloro-N-methylaniline + [reduced NADPH-hemoprotein reductase] + O2	N-demethylase activity	Helianthus tuberosus	p-chloroaniline + ? + [oxidized NADPH-hemoprotein reductase] + H2O	-	?
trans-cinnamate + [reduced NADPH-hemoprotein reductase] + O2	-	Helianthus tuberosus	4-hydroxycinnamate + [oxidized NADPH-hemoprotein reductase] + H2O	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.000233	-	chlorotoluron	-	Helianthus tuberosus
0.0567	-	7-ethoxycoumarin	-	Helianthus tuberosus
0.19	-	7-methoxycoumarin	-	Helianthus tuberosus
2.37	-	p-chloro-N-methylaniline	-	Helianthus tuberosus
4.95	-	trans-cinnamate	-	Helianthus tuberosus

Cofactor

Cofactor	Comment	Organism	Structure
cytochrome P450	-	Helianthus tuberosus

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Release 2023.1 (January 2023)