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Literature summary for 1.14.14.9 extracted from
- Catalytic activity of the two-component flavin-dependent monooxygenase from Pseudomonas aeruginosa toward cinnamic acid derivatives (2014), Appl. Microbiol. Biotechnol., 98, 1145-1154 .
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | investigation of a flask-scale production of caffeic acid from p-coumaric acid as the model reaction for HpaBC-catalyzed synthesis of hydroxycinnamic acids. Since the initial concentrations of the substrate p-coumaric acid higher than 40 mM markedly inhibits its 4-hydroxyphenylacetate 3-hydroxylases-catalyzed oxidation, the reaction is carried out by repeatedly adding 20 mM of this substrate to the reaction mixture. Furthermore, by using the whole-cell catalyst in the presence of glycerol, the experimental setup achieves the high-yield production of caffeic acid, i.e., 56.6 mM (10.2 g/l) within 24 h. These catalytic activities of 4-hydroxyphenylacetate 3-hydroxylases will provide an easy and environment-friendly synthetic approach to hydroxycinnamic acids | Pseudomonas aeruginosa |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Pseudomonas aeruginosa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| p-coumaric acid | substrate inhibition at concentrations higher than 40 mM | Pseudomonas aeruginosa |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | Q9HWT7 and Q9HWT6 | hpaB: Q9HWT7, hpaC: Q9HWT6. The enzyme consists of a reductase component HpaC and an oxygenase component HpaB | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-(3,4-dihydroxyphenyl)-propanoic acid + FADH2 + O2 | low activity | Pseudomonas aeruginosa | 3-(3,4,5-trihydroxyphenyl)-propanoic acid + FAD + H2O | - |
? | |
| 3-(4-hydroxyphenyl)-propanoic acid + FADH2 + O2 | - |
Pseudomonas aeruginosa | 3-(3,4-dihydroxyphenyl)-propanoic acid + FAD + H2O | - |
? | |
| 4-hydroxyphenylacetate + FADH2 + O2 | - |
Pseudomonas aeruginosa | 3,4-dihydroxyphenylacetate + FAD + H2O | - |
? | |
| caffeic acid + FADH2 + O2 | - |
Pseudomonas aeruginosa | 3,4,5-trihydroxycinnamic acid + FAD + H2O | - |
? | |
| coniferaldehyde + FADH2 + O2 | - |
Pseudomonas aeruginosa | 5-hydroxyconiferaldehyde + FAD + H2O | - |
? | |
| ferulic acid + FADH2 + O2 | - |
Pseudomonas aeruginosa | 5-hydroxyferulic acid + FAD + H2O | - |
? | |
| additional information | no or very low oxidation activity toward cinnamic acid, o-coumaric acid and m-coumaric acid | Pseudomonas aeruginosa | ? | - |
? | |
| p-coumaric acid + FADH2 + O2 | - |
Pseudomonas aeruginosa | caffeic acid + FAD + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 4-hydroxyphenylacetate 3-hydroxylase | - |
Pseudomonas aeruginosa |
| HPA 3-hydroxylase | - |
Pseudomonas aeruginosa |
| hpaBC | - |
Pseudomonas aeruginosa |
| HPAH | - |
Pseudomonas aeruginosa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Pseudomonas aeruginosa | |
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