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Literature summary for 1.14.14.9 extracted from

  • Thotsaporn, K.; Chenprakhon, P.; Sucharitakul, J.; Mattevi, A.; Chaiyen, P.
    Stabilization of C4a-hydroperoxyflavin in a two-component flavin-dependent monooxygenase is achieved through interactions at flavin N5 and C4a atoms (2011), J. Biol. Chem., 286, 28170-28180.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Thermus thermophilus

Protein Variants

Protein Variants Comment Organism
S171A the mutant shows reduced activity compared to the wild type enzyme Thermus thermophilus
S171A/H396V inactive Thermus thermophilus
S171T the mutant shows about wild type activity Thermus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-hydroxyphenylacetate + FMNH + O2 Thermus thermophilus
-
 3,4-dihydroxyphenylacetate + FMN + H2O
-
 ?
4-hydroxyphenylacetate + FMNH + O2 Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
 3,4-dihydroxyphenylacetate + FMN + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Thermus thermophilus
-
-
-
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-hydroxyphenylacetate + FMNH + O2
-
 Thermus thermophilus 3,4-dihydroxyphenylacetate + FMN + H2O
-
 ?
4-hydroxyphenylacetate + FMNH + O2
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 3,4-dihydroxyphenylacetate + FMN + H2O
-
 ?

Synonyms

Synonyms Comment Organism
HPAH
-
 Thermus thermophilus
p-hydroxyphenylacetate 3-hydroxylase
-
 Thermus thermophilus

Cofactor

Cofactor Comment Organism Structure
flavin dependent on Thermus thermophilus