Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Br- | retards the oxidative half-reaction | Pseudomonas putida | |
Cl- | retards the oxidative half-reaction | Pseudomonas putida | |
F- | retards the oxidative half-reaction | Pseudomonas putida | |
I- | retards the oxidative half-reaction | Pseudomonas putida | |
N3- | retards the oxidative half-reaction | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxyphenylacetate + NADH + O2 | Pseudomonas putida | - |
3,4-dihydroxyphenylacetate + NAD+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
4-hydroxyphenylacetate + FADH2 + O2 = 3,4-dihydroxyphenylacetate + FAD + H2O | two-protein enzyme: the flavoprotein catalyzes the non-productive oxidation of NADH without substrate hydroxylation and the coupling protein is an absolute requirement for productive hydroxylation. Reaction mechanism and formation of 3 flavin-oxygen intermediates are studied | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-(4-hydroxyphenyl)propionate + ? | - |
Pseudomonas putida | 3-(3,4-dihydroxy)phenylpropionate + ? | - |
? | |
4-aminophenylacetate + ? | - |
Pseudomonas putida | 4-amino-3-hydroxyphenylacetate + ? | - |
? | |
4-hydroxyphenylacetate + NADH + O2 | - |
Pseudomonas putida | 3,4-dihydroxyphenylacetate + NAD+ + H2O | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavoprotein monooxygenase, absolute requirement | Pseudomonas putida |