Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cibacron blue F3GA | - |
Pseudomonas putida | |
Co2+ | - |
Pseudomonas putida | |
Cu2+ | - |
Pseudomonas putida | |
Hg2+ | - |
Pseudomonas putida | |
iodoacetate | - |
Pseudomonas putida | |
additional information | SH-groups in the active site of the enzyme suggested | Pseudomonas putida | |
N-ethylmaleimide | - |
Pseudomonas putida | |
Zn2+ | - |
Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0042 | - |
FAD | - |
Pseudomonas putida | |
0.038 | - |
4-hydroxyphenylacetate | - |
Pseudomonas putida | |
0.041 | - |
NADH | - |
Pseudomonas putida | |
0.16 | - |
phenylacetic acid | - |
Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32500 | - |
2 * 32500, alpha2 oligomeric structure, SDS-PAGE | Pseudomonas putida |
65000 | - |
gel filtration | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxyphenylacetate + NADH + O2 | Pseudomonas putida | degradative pathway involved in the assimilation of different aromatic compounds | 3,4-dihydroxyphenylacetate + NAD+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, ion-exchange, gel filtration | Pseudomonas putida |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
4-hydroxyphenylacetate + FADH2 + O2 = 3,4-dihydroxyphenylacetate + FAD + H2O | family of external flavoprotein monooxygenases. The activity is induced by the substrates | Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
197.3 | - |
- |
Pseudomonas putida |
Storage Stability | Organism |
---|---|
-20°C, stable for at least 5 months | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phenylbutyric acid + H2 | 86% of 4-hydroxyphenylacetate activity | Pseudomonas putida | ? | - |
? | |
2-phenylpropionic acid + ? | 77% of 4-hydroxyphenylacetate activity | Pseudomonas putida | ? | - |
? | |
3,4-dihydroxyphenylacetate + FAD + H2O | - |
Pseudomonas putida | 4-hydroxyphenylacetate + FADH2 + O2 | - |
r | |
3-(4-hydroxyphenyl)propionate + ? | 83% of 4-hydroxyphenylacetate activity | Pseudomonas putida | 3-(3,4-dihydroxy)phenylpropionate + ? | - |
? | |
4-hydroxyphenylacetate + NADH + O2 | - |
Pseudomonas putida | 3,4-dihydroxyphenylacetate + NAD+ + H2O | - |
? | |
4-hydroxyphenylacetate + NADH + O2 | degradative pathway involved in the assimilation of different aromatic compounds | Pseudomonas putida | 3,4-dihydroxyphenylacetate + NAD+ + H2O | - |
? | |
additional information | broad substrate range. Several other substrates show activities below 50%. The length of the acetyl moiety is very important in substrate recognition | Pseudomonas putida | ? | - |
? | |
phenylacetic acid + ? | 100% of 4-hydroxyphenylacetate activity | Pseudomonas putida | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 32500, alpha2 oligomeric structure, SDS-PAGE | Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | required for maximum activity, external flavoprotein monooxygenase | Pseudomonas putida | |
NADH | unable to use NADPH | Pseudomonas putida |