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Literature summary for 1.14.14.9 extracted from

  • Barbour, M.G.; Bayly, R.C.
    Regulation of the meta-cleavage of 4-hydroxyphenylacetic acid by Pseudomonas putida (1977), Biochem. Biophys. Res. Commun., 76, 565-571.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
additional information hydroxylase deficient mutant P23X6 created by exposure to ethylmethane sulfonate Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-hydroxyphenylacetate + NADH + O2 Pseudomonas putida degradation of 4-hydroxyphenylacetate by inducible meta-cleavage pathway 3,4-dihydroxyphenylacetate + NAD+ + H2O
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Organism

Organism UniProt Comment Textmining
Pseudomonas putida
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mutant P23X1
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Pseudomonas putida
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P23X1, NCIB 9865
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Reaction

Reaction Comment Organism Reaction ID
4-hydroxyphenylacetate + FADH2 + O2 = 3,4-dihydroxyphenylacetate + FAD + H2O genes encoding the first four enzymes of the 4-hydroxyphenylacetic acid meta-pathway exist as two operons, one containing the hydroxylase gene while the other contains the other three Pseudomonas putida
4-hydroxyphenylacetate + FADH2 + O2 = 3,4-dihydroxyphenylacetate + FAD + H2O enzymes of 4-hydroxyphenylacetic acid meta-cleavage pathway are sythesized following at least two sequential inductive events Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
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no functional enzyme and activity detected for mutant, wild-type shows activity only if exposed to 4-hydroxyphenylacetate Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-hydroxyphenylacetate + NADH + O2 enzyme is induced by 4-hydroxyphenylacetate, no constitutive synthesis Pseudomonas putida 3,4-dihydroxyphenylacetate + NAD+ + H2O
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ir
4-hydroxyphenylacetate + NADH + O2 degradation of 4-hydroxyphenylacetate by inducible meta-cleavage pathway Pseudomonas putida 3,4-dihydroxyphenylacetate + NAD+ + H2O
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?