Protein Variants | Comment | Organism |
---|---|---|
additional information | hydroxylase deficient mutant P23X6 created by exposure to ethylmethane sulfonate | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxyphenylacetate + NADH + O2 | Pseudomonas putida | degradation of 4-hydroxyphenylacetate by inducible meta-cleavage pathway | 3,4-dihydroxyphenylacetate + NAD+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
mutant P23X1 | - |
Pseudomonas putida | - |
P23X1, NCIB 9865 | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
4-hydroxyphenylacetate + FADH2 + O2 = 3,4-dihydroxyphenylacetate + FAD + H2O | genes encoding the first four enzymes of the 4-hydroxyphenylacetic acid meta-pathway exist as two operons, one containing the hydroxylase gene while the other contains the other three | Pseudomonas putida | |
4-hydroxyphenylacetate + FADH2 + O2 = 3,4-dihydroxyphenylacetate + FAD + H2O | enzymes of 4-hydroxyphenylacetic acid meta-cleavage pathway are sythesized following at least two sequential inductive events | Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
no functional enzyme and activity detected for mutant, wild-type shows activity only if exposed to 4-hydroxyphenylacetate | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxyphenylacetate + NADH + O2 | enzyme is induced by 4-hydroxyphenylacetate, no constitutive synthesis | Pseudomonas putida | 3,4-dihydroxyphenylacetate + NAD+ + H2O | - |
ir | |
4-hydroxyphenylacetate + NADH + O2 | degradation of 4-hydroxyphenylacetate by inducible meta-cleavage pathway | Pseudomonas putida | 3,4-dihydroxyphenylacetate + NAD+ + H2O | - |
? |