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Literature summary for 1.14.14.89 extracted from

  • Gao, Y.N.; Hao, Q.H.; Zhang, H.L.; Zhou, B.; Yu, X.M.; Wang, X.L.
    Reduction of soy isoflavones by use of Escherichia coli whole-cell biocatalyst expressing isoflavone reductase under aerobic conditions (2016), Lett. Appl. Microbiol., 63, 111-116 .
    View publication on PubMed
Search for more literature for 1.14.14.89

Cloned(Commentary)

Cloned (Comment) Organism
gene dgr, recombinant enzyme expression in Escherichia coli strain BL21(DE3), aerobic bioconversion capacity of the recombinant Escherichia coli whole-cell under the optimized conditions, method evaluation and optimization, overview Slackia sp. AUH-JLC159

Protein Variants

Protein Variants Comment Organism
additional information reduction of soy isoflavones by use of Escherichia coli whole-cell biocatalyst expressing isoflavone reductase under aerobic conditions, optimization of the aerobic bioconversion conditions, overview. Addition of chloramphenicol significantly increases the bioconversion capacity of the recombinant Escherichia coli whole-cell, but addition of chloramphenicol does not increase the expression of S-DGR irrespective of whether the concentration of chloramphenicol is high. The maximal concentration of daidzein that the recombinant Escherichia coli whole-cell is able to convert efficiently is 0.8 mmol/l. The average bioconversion rate for daidzein is 85.0% and the yield of 2'-hydroxydaidzein is 0.545 mmol/l Slackia sp. AUH-JLC159

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
daidzein + [reduced NADPH-hemoprotein reductase] + O2 Slackia sp. AUH-JLC159
-
 2'-hydroxydaidzein + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
genistein + [reduced NADPH-hemoprotein reductase] + O2 Slackia sp. AUH-JLC159
-
 2'-hydroxygenistein + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Slackia sp. AUH-JLC159 A0A0U1WKA6
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
daidzein + [reduced NADPH-hemoprotein reductase] + O2
-
 Slackia sp. AUH-JLC159 2'-hydroxydaidzein + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
genistein + [reduced NADPH-hemoprotein reductase] + O2
-
 Slackia sp. AUH-JLC159 2'-hydroxygenistein + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
additional information reduction of soy isoflavones daidzein and genistein under aerobic conditions by use of the recombinant Escherichia coli whole-cell expressing isoflavone reductase Slackia sp. AUH-JLC159 ?
-
 ?

Synonyms

Synonyms Comment Organism
daidzein and genistein reductase
-
 Slackia sp. AUH-JLC159
dgr
-
 Slackia sp. AUH-JLC159
S-DGR
-
 Slackia sp. AUH-JLC159

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Slackia sp. AUH-JLC159

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Slackia sp. AUH-JLC159

Cofactor

Cofactor Comment Organism Structure
cytochrome P-450
-
 Slackia sp. AUH-JLC159
NADPH-hemoprotein reductase A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._ Slackia sp. AUH-JLC159