Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | cells susceptible to Ascochyta rabiei can be elicitor-induced to 500% activity | Cicer arietinum | |
NADH | synergistic effect with NADPH | Cicer arietinum | |
NADH | 135% of NADPH activity | Cicer arietinum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cytochrome c | 0.01 mM cytochrome c reduces activity to 14%, 0.1 mM cytochrome c reduces activity to 4% | Cicer arietinum | |
additional information | no inhibition by 5 mM KCN and 1 mM EDTA | Cicer arietinum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Cicer arietinum | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
formononetin + [reduced NADPH-hemoprotein reductase] + O2 | Cicer arietinum | involved in the biosynthesis of the pterocarpin phytoalexin medicarpin | 2'-hydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
ir | |
formononetin + [reduced NADPH-hemoprotein reductase] + O2 | Cicer arietinum | cytochrome P450-dependent monooxygenase | 2'-hydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cicer arietinum | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | suspension | Cicer arietinum | - |
cell suspension culture | - |
Cicer arietinum | - |
Storage Stability | Organism |
---|---|
-20°C, 100 mM potassium phosphate, pH 7.5 containing 400 mM sucrose and 3.5 mM 2-mercaptoethanol, 24 h | Cicer arietinum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
biochanin A + [reduced NADPH-hemoprotein reductase] + O2 | - |
Cicer arietinum | 2'-hydroxybiochanin A + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
biochanin A + [reduced NADPH-hemoprotein reductase] + O2 | - |
Cicer arietinum | 2'-hydroxybiochanin A + [oxidized NADPH-hemoprotein reductase] + H2O | - |
ir | |
daidzein + [reduced NADPH-hemoprotein reductase] + O2 | 2.0% of the activity with biochanin A | Cicer arietinum | 2'-hydroxydaidzein + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
formononetin + [reduced NADPH-hemoprotein reductase] + O2 | - |
Cicer arietinum | 2'-hydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
ir | |
formononetin + [reduced NADPH-hemoprotein reductase] + O2 | involved in the biosynthesis of the pterocarpin phytoalexin medicarpin | Cicer arietinum | 2'-hydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
ir | |
formononetin + [reduced NADPH-hemoprotein reductase] + O2 | cytochrome P450-dependent monooxygenase | Cicer arietinum | 2'-hydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
ir | |
formononetin + [reduced NADPH-hemoprotein reductase] + O2 | 78.1% of the activity with biochanin A | Cicer arietinum | 2'-hydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
genistein + [reduced NADPH-hemoprotein reductase] + O2 | 2.2% of the activity with biochanin A | Cicer arietinum | 2'-hydroxygenistein + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
additional information | monohydroxylation of 4'-methoxyisoflavones, no substrates are genistein and daidzein | Cicer arietinum | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
isoflavone 2'-hydroxylase | ambiguous | Cicer arietinum |
isoflavone 2'-monooxygenase | ambiguous | Cicer arietinum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay conditions | Cicer arietinum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no cofactors are FAD and FMN | Cicer arietinum |