Cloned (Comment) | Organism |
---|---|
expressed in Saccharomyces cerevisiae strain BJ2168 | Glycyrrhiza echinata |
Protein Variants | Comment | Organism |
---|---|---|
L371V | inactive | Glycyrrhiza echinata |
L371V/K375T | the mutant shows 3-5% activity levels compared to the wild type. The mutant produces a mixture of 95% 3beta-hydroxyflavanone and 5% flavone from (2S)-liquiritigenin | Glycyrrhiza echinata |
S310T/K375T | the P450 level of the mutant is approximately 1.5times that of wild type. The mutant shows 3-5% activity levels compared to the wild type | Glycyrrhiza echinata |
S310T/L371V/K375T | the mutant produces 100% flavone from (2S)-liquiritigenin | Glycyrrhiza echinata |
S310T/L371V/K375T | the mutant shows 4times higher P450 level than the wild type | Glycyrrhiza echinata |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycyrrhiza echinata | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S)-7,4'-dihydroxyflavanone + [reduced NADPH-hemoprotein reductase] + O2 | i.e. (2S)-liquiritigenin | Glycyrrhiza echinata | 2,7,4'-trihydroxyisoflavanone + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
2-hydroxyisoflavanone synthase | - |
Glycyrrhiza echinata |
2HI synthase | - |
Glycyrrhiza echinata |
CYP93C | - |
Glycyrrhiza echinata |
IFS | - |
Glycyrrhiza echinata |