Cloned (Comment) | Organism |
---|---|
cloned from leaf microsomes, functional recombinant expression in Saccharomyces cerevisiae | Montanoa tomentosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08032 | - |
ent-kaur-16-ene | pH 7.2, 30°C, leaf microsomes | Montanoa tomentosa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Montanoa tomentosa | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 | Montanoa tomentosa | overall reaction | ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Montanoa tomentosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme partially as leaves microsomes | Montanoa tomentosa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Montanoa tomentosa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 | overall reaction | Montanoa tomentosa | ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MtKO | - |
Montanoa tomentosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Montanoa tomentosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Montanoa tomentosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P450 | - |
Montanoa tomentosa | |
FAD | - |
Montanoa tomentosa | |
NADPH | - |
Montanoa tomentosa |