Cloned (Comment) | Organism |
---|---|
gene linC, DNA and amino acid sequence determination and analysis, constitutive functional expression in Escherichia coli from plasmid pUC18 under control of the lac promoter | Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | heme-containing cytochrome P450 enzyme | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
linalool + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 | Pseudomonas putida | - |
(6E)-8-oxolinalool + 2 [oxidized NADPH-hemoprotein reductase] + 3 H2O | - |
? | |
linalool + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 | Pseudomonas putida PpG777 | - |
(6E)-8-oxolinalool + 2 [oxidized NADPH-hemoprotein reductase] + 3 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | Q59723 | a D110R strain, gene linC | - |
Pseudomonas putida PpG777 | Q59723 | a D110R strain, gene linC | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli by anion exchange chromatography, ammonium sulfate fractionation, gel filtration, and a second step of anion exchange chromatography | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
linalool + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 | - |
Pseudomonas putida | (6E)-8-oxolinalool + 2 [oxidized NADPH-hemoprotein reductase] + 3 H2O | - |
? | |
linalool + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 | - |
Pseudomonas putida PpG777 | (6E)-8-oxolinalool + 2 [oxidized NADPH-hemoprotein reductase] + 3 H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
P45O1in | - |
Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P450 | - |
Pseudomonas putida | |
heme | - |
Pseudomonas putida |
General Information | Comment | Organism |
---|---|---|
evolution | P45O1in is a member of the CYPIll P-450 monooxygenase gene family | Pseudomonas putida |
metabolism | P-4501in catalyzes the 8-methyl hydroxylation of linalool as the first committed step of its utilization by Pseudomonas putida as the sole carbon source | Pseudomonas putida |
additional information | P-4501in exhibits conservation in the axial cysteine heme ligand-containing peptide and the threonine region postulated to form an 02-binding pocket | Pseudomonas putida |