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Literature summary for 1.14.14.84 extracted from

  • Bell, S.G.; Dale, A.; Rees, N.H.; Wong, L.L.
    A cytochrome P450 class I electron transfer system from Novosphingobium aromaticivorans (2010), Appl. Microbiol. Biotechnol., 86, 163-175.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
CYP111A2 cloning, expression in Escherichia coli strain DH5alpha Novosphingobium aromaticivorans

Protein Variants

Protein Variants Comment Organism
additional information interaction and coupling of CYP enzyme reactions from Novosphingobium aromaticivorans with ferredoxin reductases ArR from Novosphingobium aromaticivorans and PdR from from Rhodopseudomonas palustris CGA009, activity reconstitution with the electron transfer system of ArR and Arx, overview Novosphingobium aromaticivorans

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ in the heme cofactor Novosphingobium aromaticivorans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
linalool + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 Novosphingobium aromaticivorans
-
(6E)-8-oxolinalool + 2 [oxidized NADPH-hemoprotein reductase] + 3 H2O
-
?
linalool + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 Novosphingobium aromaticivorans ATCC 700278D-5
-
(6E)-8-oxolinalool + 2 [oxidized NADPH-hemoprotein reductase] + 3 H2O
-
?

Organism

Organism UniProt Comment Textmining
Novosphingobium aromaticivorans
-
-
-
Novosphingobium aromaticivorans ATCC 700278D-5
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli by ultrafiltration and anion exchange chromatography Novosphingobium aromaticivorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
linalool + 2 [reduced NADPH-hemoprotein reductase] + 2 O2
-
Novosphingobium aromaticivorans (6E)-8-oxolinalool + 2 [oxidized NADPH-hemoprotein reductase] + 3 H2O
-
?
linalool + 2 [reduced NADPH-hemoprotein reductase] + 2 O2
-
Novosphingobium aromaticivorans (6E)-8-oxolinalool + 2 [oxidized NADPH-hemoprotein reductase] + 3 H2O NMR spectroscopy product analysis ?
linalool + 2 [reduced NADPH-hemoprotein reductase] + 2 O2
-
Novosphingobium aromaticivorans ATCC 700278D-5 (6E)-8-oxolinalool + 2 [oxidized NADPH-hemoprotein reductase] + 3 H2O
-
?

Synonyms

Synonyms Comment Organism
CYP111A2
-
Novosphingobium aromaticivorans
linalool hydroxylase
-
Novosphingobium aromaticivorans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Novosphingobium aromaticivorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Novosphingobium aromaticivorans

Cofactor

Cofactor Comment Organism Structure
cytochrome P450
-
Novosphingobium aromaticivorans
heme dependent on Novosphingobium aromaticivorans

General Information

General Information Comment Organism
evolution the enzyme belongs to the CYP111 family of heme-dependent cytochrome P450 monooxygenases Novosphingobium aromaticivorans