Cloned (Comment) | Organism |
---|---|
CYP111A2 cloning, expression in Escherichia coli strain DH5alpha | Novosphingobium aromaticivorans |
Protein Variants | Comment | Organism |
---|---|---|
additional information | interaction and coupling of CYP enzyme reactions from Novosphingobium aromaticivorans with ferredoxin reductases ArR from Novosphingobium aromaticivorans and PdR from from Rhodopseudomonas palustris CGA009, activity reconstitution with the electron transfer system of ArR and Arx, overview | Novosphingobium aromaticivorans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | in the heme cofactor | Novosphingobium aromaticivorans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
linalool + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 | Novosphingobium aromaticivorans | - |
(6E)-8-oxolinalool + 2 [oxidized NADPH-hemoprotein reductase] + 3 H2O | - |
? | |
linalool + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 | Novosphingobium aromaticivorans ATCC 700278D-5 | - |
(6E)-8-oxolinalool + 2 [oxidized NADPH-hemoprotein reductase] + 3 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Novosphingobium aromaticivorans | - |
- |
- |
Novosphingobium aromaticivorans ATCC 700278D-5 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli by ultrafiltration and anion exchange chromatography | Novosphingobium aromaticivorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
linalool + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 | - |
Novosphingobium aromaticivorans | (6E)-8-oxolinalool + 2 [oxidized NADPH-hemoprotein reductase] + 3 H2O | - |
? | |
linalool + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 | - |
Novosphingobium aromaticivorans | (6E)-8-oxolinalool + 2 [oxidized NADPH-hemoprotein reductase] + 3 H2O | NMR spectroscopy product analysis | ? | |
linalool + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 | - |
Novosphingobium aromaticivorans ATCC 700278D-5 | (6E)-8-oxolinalool + 2 [oxidized NADPH-hemoprotein reductase] + 3 H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYP111A2 | - |
Novosphingobium aromaticivorans |
linalool hydroxylase | - |
Novosphingobium aromaticivorans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Novosphingobium aromaticivorans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Novosphingobium aromaticivorans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P450 | - |
Novosphingobium aromaticivorans | |
heme | dependent on | Novosphingobium aromaticivorans |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the CYP111 family of heme-dependent cytochrome P450 monooxygenases | Novosphingobium aromaticivorans |