Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant enzyme overexpression in Petunia hybrida hybrid M1 x R27, which contains a mutated endogenous F3'5'H (HF1), no obvious phenotype | Phalaenopsis sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydrokaempferol + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 | Phalaenopsis sp. | overall reaction | dihydromyricetin + 2 [oxidized NADPH-hemoprotein reductase] + 2 H2O | - |
? | |
dihydrokaempferol + [reduced NADPH-hemoprotein reductase] + O2 | Phalaenopsis sp. | - |
dihydroquercetin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
dihydroquercetin + [reduced NADPH-hemoprotein reductase] + O2 | Phalaenopsis sp. | - |
dihydromyricetin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phalaenopsis hybrid cultivar | Q3YAF0 | cv. Big Chili | - |
Phalaenopsis sp. | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower bud | weak expression at stages 1-3 of bud development | Phalaenopsis hybrid cultivar | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydrokaempferol + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 | overall reaction | Phalaenopsis sp. | dihydromyricetin + 2 [oxidized NADPH-hemoprotein reductase] + 2 H2O | - |
? | |
dihydrokaempferol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Phalaenopsis sp. | dihydroquercetin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
dihydroquercetin + [reduced NADPH-hemoprotein reductase] + O2 | - |
Phalaenopsis sp. | dihydromyricetin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
F3'5'H | - |
Phalaenopsis sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Phalaenopsis sp. |
General Information | Comment | Organism |
---|---|---|
evolution | the F3'5'H gene in Phalaenopsis has a relatively large evolutionary distance to other plant F3'5'Hs | Phalaenopsis sp. |
physiological function | overexpression in petunia shows that it has no function in anthocyanin production | Phalaenopsis hybrid cultivar |
physiological function | Phalaenopsis genome contains the F3'5'H gene, which is the key gene for violet/blue colour, but the enzyme has no function in anthocyanin production | Phalaenopsis sp. |