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Literature summary for 1.14.14.81 extracted from

  • Wang, L.; Zhang, J.; Dong, X.; Fu, Z.; Jiang, H.; Zhang, H.
    Identification and functional analysis of anthocyanin biosynthesis genes in Phalaenopsis hybrids (2018), Biol. Plant., 62, 45-54 .
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant enzyme overexpression in Petunia hybrida hybrid M1 x R27, which contains a mutated endogenous F3'5'H (HF1), no obvious phenotype Phalaenopsis sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dihydrokaempferol + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 Phalaenopsis sp. overall reaction dihydromyricetin + 2 [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
?
dihydrokaempferol + [reduced NADPH-hemoprotein reductase] + O2 Phalaenopsis sp.
-
dihydroquercetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
dihydroquercetin + [reduced NADPH-hemoprotein reductase] + O2 Phalaenopsis sp.
-
dihydromyricetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Organism

Organism UniProt Comment Textmining
Phalaenopsis hybrid cultivar Q3YAF0 cv. Big Chili
-
Phalaenopsis sp.
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
flower bud weak expression at stages 1-3 of bud development Phalaenopsis hybrid cultivar
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dihydrokaempferol + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 overall reaction Phalaenopsis sp. dihydromyricetin + 2 [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
?
dihydrokaempferol + [reduced NADPH-hemoprotein reductase] + O2
-
Phalaenopsis sp. dihydroquercetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
dihydroquercetin + [reduced NADPH-hemoprotein reductase] + O2
-
Phalaenopsis sp. dihydromyricetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Synonyms

Synonyms Comment Organism
F3'5'H
-
Phalaenopsis sp.

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Phalaenopsis sp.

General Information

General Information Comment Organism
evolution the F3'5'H gene in Phalaenopsis has a relatively large evolutionary distance to other plant F3'5'Hs Phalaenopsis sp.
physiological function overexpression in petunia shows that it has no function in anthocyanin production Phalaenopsis hybrid cultivar
physiological function Phalaenopsis genome contains the F3'5'H gene, which is the key gene for violet/blue colour, but the enzyme has no function in anthocyanin production Phalaenopsis sp.