Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | maximal activation at 20 mM | Petunia x hybrida |
General Stability | Organism |
---|---|
activity declines when microsomal suspensions are subjected to freezing and thawing | Petunia x hybrida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-aminobenzotriazole | - |
Petunia x hybrida | |
CO | - |
Petunia x hybrida | |
cytochrome c | 0.05 mM, complete inhibition | Petunia x hybrida | |
decyl-beta-D-glucopyranoside | dose-dependent inhibition | Petunia x hybrida | |
diethyldithiocarbamate | - |
Petunia x hybrida | |
Digitonin | dose-dependent inhibition | Petunia x hybrida | |
dodecyl maltoside | dose-dependent inhibition | Petunia x hybrida | |
Emulgen 911 | dose-dependent inhibition | Petunia x hybrida | |
Emulgen 913 | dose-dependent inhibition | Petunia x hybrida | |
heptyl-beta-D-glucopyranoside | dose-dependent inhibition | Petunia x hybrida | |
hexyl-beta-D-glucopyranoside | dose-dependent inhibition | Petunia x hybrida | |
Lubrol Px | dose-dependent inhibition | Petunia x hybrida | |
Mega-10 | dose-dependent inhibition | Petunia x hybrida | |
Mega-8 | dose-dependent inhibition | Petunia x hybrida | |
Mega-9 | dose-dependent inhibition | Petunia x hybrida | |
additional information | a polyclonal antibody that inhibits higher plant NADPH-cytochrome p450 reductase inhibits the flavonoid 3',5'-hydroxylase. No effect by diethylpyrocarbonate or phenylmethylsulfonyl fluoride | Petunia x hybrida | |
NEM | - |
Petunia x hybrida | |
Nonidet P40 | dose-dependent inhibition | Petunia x hybrida | |
nonyl-beta-D-glucopyranoside | dose-dependent inhibition | Petunia x hybrida | |
octyl-beta-D-glucopyranoside | dose-dependent inhibition | Petunia x hybrida | |
sodium cholate | dose-dependent inhibition | Petunia x hybrida | |
sodium deoxycholate | dose-dependent inhibition | Petunia x hybrida | |
Tetcyclacis | - |
Petunia x hybrida | |
Triton X-100 | dose-dependent inhibition | Petunia x hybrida | |
Triton X-114 | dose-dependent inhibition | Petunia x hybrida | |
Tween 20 | dose-dependent inhibition | Petunia x hybrida | |
Tween 80 | dose-dependent inhibition | Petunia x hybrida | |
Zwittergent | dose-dependent inhibition | Petunia x hybrida | |
Zwittergent 3-08 | dose-dependent inhibition | Petunia x hybrida | |
Zwittergent 3-10 | dose-dependent inhibition | Petunia x hybrida | |
Zwittergent 3-12 | dose-dependent inhibition | Petunia x hybrida | |
Zwittergent 3-14 | dose-dependent inhibition | Petunia x hybrida | |
Zwittergent 3-16 | dose-dependent inhibition | Petunia x hybrida |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | microsomal membrane | Petunia x hybrida | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Petunia x hybrida | - |
cv. Old Glory Blue | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower bud | activity varies with the development of flowers, peaking immediately prior to and during anthesis, but is absent in mature flowers | Petunia x hybrida | - |
Storage Stability | Organism |
---|---|
4°C, stable overnight in presence of 20% glycerol or sucrose at pH 7.5 | Petunia x hybrida |
75°C, storage in presence of 20% sucrose for periods of greater than 1 month results in significant loss of enzyme activity | Petunia x hybrida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,7,3',4'-tetrahydroxyflavanone + [reduced NADPH-hemoprotein reductase] + O2 | - |
Petunia x hybrida | 5,7,3',4',5'-pentahydroxyflavanone + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
5,7,4'-trihydroxyflavanone + [reduced NADPH-hemoprotein reductase] + O2 | - |
Petunia x hybrida | 5,7,3',4'-tetrahydroxyflavanone + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
dihydroquercetin + [reduced NADPH-hemoprotein reductase] + O2 | - |
Petunia x hybrida | 5'-hydroxydihydroquercetin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
naringenin + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 | - |
Petunia x hybrida | (2S)-5,7,3',4',5'-pentahydroxyflavanone + 2 [oxidized NADPH-hemoprotein reductase] + 2 H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
F3',5'H | - |
Petunia x hybrida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Petunia x hybrida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P450 | inhibition by a polyclonal antibody that inhibits higher plant NADPH-cytochrome p450 reductase is consistent with the suggestion that flavonoid 3',5'-hydroxylase is a monooxygenase consisting of cytochrome p450 and a NADPH-cytochrome P-450 reductase | Petunia x hybrida |