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Literature summary for 1.14.14.81 extracted from

  • Menting, J.G.T.; Scopes, R.K.; Stevenson, T.W.
    Characterization of flavonoid 3',5'-hydroxylase in microsomal membrane fraction of Petunia hybrida flowers (1994), Plant Physiol., 106, 633-642.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol maximal activation at 20 mM Petunia x hybrida

General Stability

General Stability Organism
activity declines when microsomal suspensions are subjected to freezing and thawing Petunia x hybrida

Inhibitors

Inhibitors Comment Organism Structure
1-aminobenzotriazole
-
Petunia x hybrida
CO
-
Petunia x hybrida
cytochrome c 0.05 mM, complete inhibition Petunia x hybrida
decyl-beta-D-glucopyranoside dose-dependent inhibition Petunia x hybrida
diethyldithiocarbamate
-
Petunia x hybrida
Digitonin dose-dependent inhibition Petunia x hybrida
dodecyl maltoside dose-dependent inhibition Petunia x hybrida
Emulgen 911 dose-dependent inhibition Petunia x hybrida
Emulgen 913 dose-dependent inhibition Petunia x hybrida
heptyl-beta-D-glucopyranoside dose-dependent inhibition Petunia x hybrida
hexyl-beta-D-glucopyranoside dose-dependent inhibition Petunia x hybrida
Lubrol Px dose-dependent inhibition Petunia x hybrida
Mega-10 dose-dependent inhibition Petunia x hybrida
Mega-8 dose-dependent inhibition Petunia x hybrida
Mega-9 dose-dependent inhibition Petunia x hybrida
additional information a polyclonal antibody that inhibits higher plant NADPH-cytochrome p450 reductase inhibits the flavonoid 3',5'-hydroxylase. No effect by diethylpyrocarbonate or phenylmethylsulfonyl fluoride Petunia x hybrida
NEM
-
Petunia x hybrida
Nonidet P40 dose-dependent inhibition Petunia x hybrida
nonyl-beta-D-glucopyranoside dose-dependent inhibition Petunia x hybrida
octyl-beta-D-glucopyranoside dose-dependent inhibition Petunia x hybrida
sodium cholate dose-dependent inhibition Petunia x hybrida
sodium deoxycholate dose-dependent inhibition Petunia x hybrida
Tetcyclacis
-
Petunia x hybrida
Triton X-100 dose-dependent inhibition Petunia x hybrida
Triton X-114 dose-dependent inhibition Petunia x hybrida
Tween 20 dose-dependent inhibition Petunia x hybrida
Tween 80 dose-dependent inhibition Petunia x hybrida
Zwittergent dose-dependent inhibition Petunia x hybrida
Zwittergent 3-08 dose-dependent inhibition Petunia x hybrida
Zwittergent 3-10 dose-dependent inhibition Petunia x hybrida
Zwittergent 3-12 dose-dependent inhibition Petunia x hybrida
Zwittergent 3-14 dose-dependent inhibition Petunia x hybrida
Zwittergent 3-16 dose-dependent inhibition Petunia x hybrida

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome microsomal membrane Petunia x hybrida
-
-

Organism

Organism UniProt Comment Textmining
Petunia x hybrida
-
cv. Old Glory Blue
-

Source Tissue

Source Tissue Comment Organism Textmining
flower bud activity varies with the development of flowers, peaking immediately prior to and during anthesis, but is absent in mature flowers Petunia x hybrida
-

Storage Stability

Storage Stability Organism
4°C, stable overnight in presence of 20% glycerol or sucrose at pH 7.5 Petunia x hybrida
75°C, storage in presence of 20% sucrose for periods of greater than 1 month results in significant loss of enzyme activity Petunia x hybrida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,7,3',4'-tetrahydroxyflavanone + [reduced NADPH-hemoprotein reductase] + O2
-
Petunia x hybrida 5,7,3',4',5'-pentahydroxyflavanone + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
5,7,4'-trihydroxyflavanone + [reduced NADPH-hemoprotein reductase] + O2
-
Petunia x hybrida 5,7,3',4'-tetrahydroxyflavanone + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
dihydroquercetin + [reduced NADPH-hemoprotein reductase] + O2
-
Petunia x hybrida 5'-hydroxydihydroquercetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
naringenin + 2 [reduced NADPH-hemoprotein reductase] + 2 O2
-
Petunia x hybrida (2S)-5,7,3',4',5'-pentahydroxyflavanone + 2 [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
?

Synonyms

Synonyms Comment Organism
F3',5'H
-
Petunia x hybrida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Petunia x hybrida

Cofactor

Cofactor Comment Organism Structure
cytochrome P450 inhibition by a polyclonal antibody that inhibits higher plant NADPH-cytochrome p450 reductase is consistent with the suggestion that flavonoid 3',5'-hydroxylase is a monooxygenase consisting of cytochrome p450 and a NADPH-cytochrome P-450 reductase Petunia x hybrida