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Literature summary for 1.14.14.81 extracted from

  • Shimada, Y.; Nakano-Shimada, R.; Ohbayashi, M.; Okinaka, Y.; Kiyokawa, S.; Kikuchi, Y.
    Expression of chimeric P450 genes encoding flavonoid-3', 5'-hydroxylase in transgenic tobacco and petunia plants(1) (1999), FEBS Lett., 461, 241-245.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
subcloning of AK14, encoding flavonoid-3',5'-hydroxylase, into a plant expression vector and transforming it to pink tobacco (Nicotiana tabacum cv. Petit Havana SR1) and pink petunia (var. Falcon), both of which originally lack the enzyme Petunia sp.
subcloning of TG1, encoding flavonoid-3',5'-hydroxylase, into a plant expression vector and transforming it to pink tobacco (Nicotiana tabacum cv. Petit Havana SR1) which originally lacks the enzyme Eustoma grandiflorum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Petunia x hybrida key enzyme in the synthesis of 3',5'-hydroxylated anthocyanins, which are generally required for blue or purple flowers ?
-
 ?
additional information Eustoma grandiflorum key enzyme in the synthesis of 3',5'-hydroxylated anthocyanins, which are generally required for blue or purple flowers ?
-
 ?

Organism

Organism UniProt Comment Textmining
Eustoma grandiflorum O04790 Lisianthus russelianus
-
Petunia x hybrida P48418
-
-

Source Tissue

Source Tissue Comment Organism Textmining
flower bud
-
 Eustoma grandiflorum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information key enzyme in the synthesis of 3',5'-hydroxylated anthocyanins, which are generally required for blue or purple flowers Petunia x hybrida ?
-
 ?
additional information key enzyme in the synthesis of 3',5'-hydroxylated anthocyanins, which are generally required for blue or purple flowers Eustoma grandiflorum ?
-
 ?

Cofactor

Cofactor Comment Organism Structure
cytochrome P450 the enzyme is a member of the cytochrome P450 family Petunia x hybrida
cytochrome P450 the enzyme is a member of the cytochrome P450 family Eustoma grandiflorum