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Literature summary for 1.14.14.80 extracted from

  • Uehara, S.; Uno, Y.; Ishii, S.; Inoue, T.; Sasaki, E.; Yamazaki, H.
    Marmoset cytochrome P450 4A11, a novel arachidonic acid and lauric acid omega-hydroxylase expressed in liver and kidney tissues (2017), Xenobiotica, 47, 553-561 .
    View publication on PubMed
Search for more literature for 1.14.14.80

Cloned(Commentary)

Cloned (Comment) Organism
gene CYPP4504A11, located on chromosome 7, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, real-time RT PCR enzyme expression analysis, recombinant expression in Escherichia coli Callithrix jacchus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Callithrix jacchus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ in heme Callithrix jacchus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
arachidonic acid + [reduced NADPH-hemoprotein reductase] + O2 Callithrix jacchus
-
 20-hydroxyarachidonic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
lauric acid + [reduced NADPH-hemoprotein reductase] + O2 Callithrix jacchus
-
 12-hydroxylauric acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
additional information Callithrix jacchus Marmoset CYP4A11 enzyme heterologously expressed in Escherichia coli preferentially catalyzes the omega-hydroxylation of arachidonic acid and lauric acid, similar to enzymes from Macaca fascicularis and Homo sapiens. The lauric acid omega-hydroxylation activity of marmoset CYP4A11 is low compared with those of marmoset liver microsomes ?
-
 ?

Organism

Organism UniProt Comment Textmining
Callithrix jacchus A0A1P8NQJ7 white-tufted-ear marmoset
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Source Tissue

Source Tissue Comment Organism Textmining
kidney highest expression level Callithrix jacchus
-
liver
-
 Callithrix jacchus
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additional information expression pattern by real-time RT PCR and immunoblottig. No expression in brains, lungs, and small intestines Callithrix jacchus
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arachidonic acid + [reduced NADPH-hemoprotein reductase] + O2
-
 Callithrix jacchus 20-hydroxyarachidonic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
lauric acid + [reduced NADPH-hemoprotein reductase] + O2
-
 Callithrix jacchus 12-hydroxylauric acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
additional information Marmoset CYP4A11 enzyme heterologously expressed in Escherichia coli preferentially catalyzes the omega-hydroxylation of arachidonic acid and lauric acid, similar to enzymes from Macaca fascicularis and Homo sapiens. The lauric acid omega-hydroxylation activity of marmoset CYP4A11 is low compared with those of marmoset liver microsomes Callithrix jacchus ?
-
 ?
additional information the enzyme sequence contains consensus sequences of six substrate recognition sites. Marmoset CYP4A11 enzyme heterologously expressed in Escherichia coli preferentially catalyzes the omega-hydroxylation of arachidonic acid and lauric acid, similar to enzymes from Macaca fascicularis and Homo sapiens. The lauric acid omega-hydroxylation activity of marmoset CYP4A11 is low compared with those of marmoset liver microsomes. CYP4A11 hydroxylates fatty acids at their omega and omega-1 positions Callithrix jacchus ?
-
 ?

Synonyms

Synonyms Comment Organism
CYP4A11
-
 Callithrix jacchus
Fatty acid omega-hydroxylase
-
 Callithrix jacchus
omega-hydroxylase
-
 Callithrix jacchus
P450 4A11
-
 Callithrix jacchus

Cofactor

Cofactor Comment Organism Structure
cytochrome P450
-
 Callithrix jacchus
heme the enzyme contains one heme-binding domain Callithrix jacchus
NADPH
-
 Callithrix jacchus