BRENDA - Enzyme Database show
show all sequences of 1.14.14.8

Characterization of the anthranilate degradation pathway in Geobacillus thermodenitrificans NG80-2

Liu, X.; Dong, Y.; Li, X.; Ren, Y.; Li, Y.; Wang, W.; Wang, L.; Feng, L.; Microbiology 156, 589-595 (2010)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli; expression in Escherichia coli
Geobacillus thermodenitrificans
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Geobacillus thermodenitrificans
-
-
-
Geobacillus thermodenitrificans NG80-2
-
-
-
Purification (Commentary)
Commentary
Organism
using Ni-NTA chromatography
Geobacillus thermodenitrificans
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
151.3
-
substrate anthranilate, pH 9.0, 60°C
Geobacillus thermodenitrificans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-hydroxyphenylacetate + FADH2 + O2
67.8% of the activity with anthranilate
709852
Geobacillus thermodenitrificans
? + FAD + H2O
-
-
-
?
2-hydroxyphenylacetate + FADH2 + O2
67.8% of the activity with anthranilate
709852
Geobacillus thermodenitrificans NG80-2
? + FAD + H2O
-
-
-
?
2-hydroxyphenylacetate + FADH2 + O2
62.79% relative activity
709852
Geobacillus thermodenitrificans
?
-
-
-
?
4-hydroxyphenylacetate + FADH2 + O2
42.7% of the activity with anthranilate
709852
Geobacillus thermodenitrificans
? + FAD + H2O
-
-
-
?
4-hydroxyphenylacetate + FADH2 + O2
42.7% of the activity with anthranilate
709852
Geobacillus thermodenitrificans NG80-2
? + FAD + H2O
-
-
-
?
4-hydroxyphenylacetate + FADH2 + O2
42.7% relative activity
709852
Geobacillus thermodenitrificans
?
-
-
-
?
anthranilate + FADH2 + O2
-
709852
Geobacillus thermodenitrificans
3-hydroxyanthranilate + FAD + H2O
-
-
-
?
anthranilate + FADH2 + O2
-
709852
Geobacillus thermodenitrificans NG80-2
3-hydroxyanthranilate + FAD + H2O
-
-
-
?
anthranilate + FADH2 + O2
highest activity
709852
Geobacillus thermodenitrificans
3-hydroxyanthranilate + FAD
-
-
-
?
additional information
no substrate: kynurenine, phenol, 2-nitrobenzylic acid, chlorobenzene, naphthalene, naphthol, toluene or ethylbenzene
709852
Geobacillus thermodenitrificans
?
-
-
-
-
additional information
no substrate: kynurenine, phenol, 2-nitrobenzylic acid, chlorobenzene, naphthalene, naphthol, toluene or ethylbenzene
709852
Geobacillus thermodenitrificans NG80-2
?
-
-
-
-
salicylate + FADH2 + O2
49.6% of the activity with anthranilate
709852
Geobacillus thermodenitrificans
? + FAD + H2O
-
-
-
?
salicylate + FADH2 + O2
49.6% of the activity with anthranilate
709852
Geobacillus thermodenitrificans NG80-2
? + FAD + H2O
-
-
-
?
salicylate + FADH2 + O2
49% relative activity
709852
Geobacillus thermodenitrificans
?
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
60
-
; assay at, highest activity
Geobacillus thermodenitrificans
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
25
70
-
Geobacillus thermodenitrificans
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
; assay at, highest activity
Geobacillus thermodenitrificans
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5
11
-
Geobacillus thermodenitrificans
Cofactor
Cofactor
Commentary
Organism
Structure
FADH2
; FADH2-utilizing monooxygenase, no reaction with FMNH2
Geobacillus thermodenitrificans
additional information
no cofactor: FMNH2
Geobacillus thermodenitrificans
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli; expression in Escherichia coli
Geobacillus thermodenitrificans
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FADH2
; FADH2-utilizing monooxygenase, no reaction with FMNH2
Geobacillus thermodenitrificans
additional information
no cofactor: FMNH2
Geobacillus thermodenitrificans
Purification (Commentary) (protein specific)
Commentary
Organism
using Ni-NTA chromatography
Geobacillus thermodenitrificans
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
151.3
-
substrate anthranilate, pH 9.0, 60°C
Geobacillus thermodenitrificans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-hydroxyphenylacetate + FADH2 + O2
67.8% of the activity with anthranilate
709852
Geobacillus thermodenitrificans
? + FAD + H2O
-
-
-
?
2-hydroxyphenylacetate + FADH2 + O2
67.8% of the activity with anthranilate
709852
Geobacillus thermodenitrificans NG80-2
? + FAD + H2O
-
-
-
?
2-hydroxyphenylacetate + FADH2 + O2
62.79% relative activity
709852
Geobacillus thermodenitrificans
?
-
-
-
?
4-hydroxyphenylacetate + FADH2 + O2
42.7% of the activity with anthranilate
709852
Geobacillus thermodenitrificans
? + FAD + H2O
-
-
-
?
4-hydroxyphenylacetate + FADH2 + O2
42.7% of the activity with anthranilate
709852
Geobacillus thermodenitrificans NG80-2
? + FAD + H2O
-
-
-
?
4-hydroxyphenylacetate + FADH2 + O2
42.7% relative activity
709852
Geobacillus thermodenitrificans
?
-
-
-
?
anthranilate + FADH2 + O2
-
709852
Geobacillus thermodenitrificans
3-hydroxyanthranilate + FAD + H2O
-
-
-
?
anthranilate + FADH2 + O2
-
709852
Geobacillus thermodenitrificans NG80-2
3-hydroxyanthranilate + FAD + H2O
-
-
-
?
anthranilate + FADH2 + O2
highest activity
709852
Geobacillus thermodenitrificans
3-hydroxyanthranilate + FAD
-
-
-
?
additional information
no substrate: kynurenine, phenol, 2-nitrobenzylic acid, chlorobenzene, naphthalene, naphthol, toluene or ethylbenzene
709852
Geobacillus thermodenitrificans
?
-
-
-
-
additional information
no substrate: kynurenine, phenol, 2-nitrobenzylic acid, chlorobenzene, naphthalene, naphthol, toluene or ethylbenzene
709852
Geobacillus thermodenitrificans NG80-2
?
-
-
-
-
salicylate + FADH2 + O2
49.6% of the activity with anthranilate
709852
Geobacillus thermodenitrificans
? + FAD + H2O
-
-
-
?
salicylate + FADH2 + O2
49.6% of the activity with anthranilate
709852
Geobacillus thermodenitrificans NG80-2
? + FAD + H2O
-
-
-
?
salicylate + FADH2 + O2
49% relative activity
709852
Geobacillus thermodenitrificans
?
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
60
-
; assay at, highest activity
Geobacillus thermodenitrificans
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
25
70
-
Geobacillus thermodenitrificans
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
; assay at, highest activity
Geobacillus thermodenitrificans
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5
11
-
Geobacillus thermodenitrificans
Expression
Organism
Commentary
Expression
Geobacillus thermodenitrificans
transcription level increases highly when anthranilate is used as the sole carbon source
up
Expression (protein specific)
Organism
Commentary
Expression
Geobacillus thermodenitrificans
transcription level increases highly when anthranilate is used as the sole carbon source
up
Other publictions for EC 1.14.14.8
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
709852
Liu
Characterization of the anthra ...
Geobacillus thermodenitrificans, Geobacillus thermodenitrificans NG80-2
Microbiology
156
589-595
2010
-
-
1
-
-
-
-
-
-
-
-
-
-
9
-
-
1
-
-
-
1
-
14
-
1
1
-
-
1
1
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
14
-
1
1
-
-
1
1
-
-
1
-
-
1
-
-