Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Geobacillus thermodenitrificans |
expression in Escherichia coli | Geobacillus thermodenitrificans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus thermodenitrificans | - |
- |
- |
Geobacillus thermodenitrificans NG80-2 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Geobacillus thermodenitrificans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
151.3 | - |
substrate anthranilate, pH 9.0, 60°C | Geobacillus thermodenitrificans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hydroxyphenylacetate + FADH2 + O2 | 67.8% of the activity with anthranilate | Geobacillus thermodenitrificans | ? + FAD + H2O | - |
? | |
2-hydroxyphenylacetate + FADH2 + O2 | 67.8% of the activity with anthranilate | Geobacillus thermodenitrificans NG80-2 | ? + FAD + H2O | - |
? | |
2-hydroxyphenylacetate + FADH2 + O2 | 62.79% relative activity | Geobacillus thermodenitrificans | ? | - |
? | |
4-hydroxyphenylacetate + FADH2 + O2 | 42.7% of the activity with anthranilate | Geobacillus thermodenitrificans | ? + FAD + H2O | - |
? | |
4-hydroxyphenylacetate + FADH2 + O2 | 42.7% of the activity with anthranilate | Geobacillus thermodenitrificans NG80-2 | ? + FAD + H2O | - |
? | |
4-hydroxyphenylacetate + FADH2 + O2 | 42.7% relative activity | Geobacillus thermodenitrificans | ? | - |
? | |
anthranilate + FADH2 + O2 | - |
Geobacillus thermodenitrificans | 3-hydroxyanthranilate + FAD + H2O | - |
? | |
anthranilate + FADH2 + O2 | - |
Geobacillus thermodenitrificans NG80-2 | 3-hydroxyanthranilate + FAD + H2O | - |
? | |
anthranilate + FADH2 + O2 | highest activity | Geobacillus thermodenitrificans | 3-hydroxyanthranilate + FAD | - |
? | |
additional information | no substrate: kynurenine, phenol, 2-nitrobenzylic acid, chlorobenzene, naphthalene, naphthol, toluene or ethylbenzene | Geobacillus thermodenitrificans | ? | - |
? | |
additional information | no substrate: kynurenine, phenol, 2-nitrobenzylic acid, chlorobenzene, naphthalene, naphthol, toluene or ethylbenzene | Geobacillus thermodenitrificans NG80-2 | ? | - |
? | |
salicylate + FADH2 + O2 | 49.6% of the activity with anthranilate | Geobacillus thermodenitrificans | ? + FAD + H2O | - |
? | |
salicylate + FADH2 + O2 | 49.6% of the activity with anthranilate | Geobacillus thermodenitrificans NG80-2 | ? + FAD + H2O | - |
? | |
salicylate + FADH2 + O2 | 49% relative activity | Geobacillus thermodenitrificans | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FADH2-utilizing monooxygenase | - |
Geobacillus thermodenitrificans |
GTNG_3160 | - |
Geobacillus thermodenitrificans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Geobacillus thermodenitrificans |
60 | - |
assay at, highest activity | Geobacillus thermodenitrificans |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 70 | - |
Geobacillus thermodenitrificans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Geobacillus thermodenitrificans |
9 | - |
assay at, highest activity | Geobacillus thermodenitrificans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 11 | - |
Geobacillus thermodenitrificans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FADH2 | - |
Geobacillus thermodenitrificans | |
FADH2 | FADH2-utilizing monooxygenase, no reaction with FMNH2 | Geobacillus thermodenitrificans | |
additional information | no cofactor: FMNH2 | Geobacillus thermodenitrificans |
Organism | Comment | Expression |
---|---|---|
Geobacillus thermodenitrificans | transcription level increases highly when anthranilate is used as the sole carbon source | up |