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Literature summary for 1.14.14.78 extracted from

  • Tang, Z.; Salamanca-Pinzon, S.; Wu, Z.; Xiao, Y.; Guengerich, F.
    Human cytochrome P450 4F11: Heterologous expression in bacteria, purification, and characterization of catalytic function (2010), Arch. Biochem. Biophys., 494, 86-93.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.047
-
palmitic acid at pH 7.4 and 37°C Homo sapiens
0.074
-
3-hydroxypalmitate at pH 7.4 and 37°C Homo sapiens
0.08
-
arachidonic acid at pH 7.4 and 37°C Homo sapiens
0.085
-
oleic acid at pH 7.4 and 37°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ contains Fe2+ Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
x * 50000, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-NTA agarose column chromatography, Biogel HTP hydroxylapatite column chromatography, and aminopropyl SPE column chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxypalmitate + [reduced NADPH-hemoprotein reductase] + O2
-
Homo sapiens 3,16-dihydroxypalmitate + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
arachidonic acid + [reduced NADPH-hemoprotein reductase] + O2
-
Homo sapiens ?
-
?
oleic acid + [reduced NADPH-hemoprotein reductase] + O2
-
Homo sapiens 18-hydroxyoleic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
palmitic acid + [reduced NADPH-hemoprotein reductase] + O2
-
Homo sapiens 16-hydroxypalmitic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Subunits

Subunits Comment Organism
? x * 50000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
cytochrome P450 4F11
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.009
-
arachidonic acid at pH 7.4 and 37°C Homo sapiens
0.02
-
oleic acid at pH 7.4 and 37°C Homo sapiens
0.03
-
palmitic acid at pH 7.4 and 37°C Homo sapiens
0.458
-
3-hydroxypalmitate at pH 7.4 and 37°C Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.12
-
arachidonic acid at pH 7.4 and 37°C Homo sapiens
0.2
-
oleic acid at pH 7.4 and 37°C Homo sapiens
0.63
-
palmitic acid at pH 7.4 and 37°C Homo sapiens
6.17
-
3-hydroxypalmitate at pH 7.4 and 37°C Homo sapiens