Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 cells | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.047 | - |
palmitic acid | at pH 7.4 and 37°C | Homo sapiens | |
0.074 | - |
3-hydroxypalmitate | at pH 7.4 and 37°C | Homo sapiens | |
0.08 | - |
arachidonic acid | at pH 7.4 and 37°C | Homo sapiens | |
0.085 | - |
oleic acid | at pH 7.4 and 37°C | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | contains Fe2+ | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
x * 50000, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-NTA agarose column chromatography, Biogel HTP hydroxylapatite column chromatography, and aminopropyl SPE column chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxypalmitate + [reduced NADPH-hemoprotein reductase] + O2 | - |
Homo sapiens | 3,16-dihydroxypalmitate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
arachidonic acid + [reduced NADPH-hemoprotein reductase] + O2 | - |
Homo sapiens | ? | - |
? | |
oleic acid + [reduced NADPH-hemoprotein reductase] + O2 | - |
Homo sapiens | 18-hydroxyoleic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
palmitic acid + [reduced NADPH-hemoprotein reductase] + O2 | - |
Homo sapiens | 16-hydroxypalmitic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 50000, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
cytochrome P450 4F11 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
arachidonic acid | at pH 7.4 and 37°C | Homo sapiens | |
0.02 | - |
oleic acid | at pH 7.4 and 37°C | Homo sapiens | |
0.03 | - |
palmitic acid | at pH 7.4 and 37°C | Homo sapiens | |
0.458 | - |
3-hydroxypalmitate | at pH 7.4 and 37°C | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
arachidonic acid | at pH 7.4 and 37°C | Homo sapiens | |
0.2 | - |
oleic acid | at pH 7.4 and 37°C | Homo sapiens | |
0.63 | - |
palmitic acid | at pH 7.4 and 37°C | Homo sapiens | |
6.17 | - |
3-hydroxypalmitate | at pH 7.4 and 37°C | Homo sapiens |