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Literature summary for 1.14.14.68 extracted from
- Optimization of recombinant expression enables discovery of novel cytochrome P450 activity in rice diterpenoid biosynthesis (2015), Appl. Microbiol. Biotechnol., 99, 7549-7558 .
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.027 | - |
9beta-pimara-7,15-diene | pH 7.5, 32°C | Oryza sativa |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryza sativa | Q0DBF4 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 9beta-pimara-7,15-diene + [reduced NADPH-hemoprotein reductase] + O2 | - |
Oryza sativa | 9beta-pimara-7,15-diene-3beta-ol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CYP701A8 | - |
Oryza sativa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 32 | - |
assay at | Oryza sativa |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.4 | - |
9beta-pimara-7,15-diene | pH 7.5, 32°C | Oryza sativa | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Oryza sativa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome P450 | - |
Oryza sativa |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 52 | - |
9beta-pimara-7,15-diene | pH 7.5, 32°C | Oryza sativa | |
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Release 2023.1 (January 2023)
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