Literature summary for 1.14.14.59 extracted from

Lee, S.; Badieyan, S.; Bevan, D.R.; Herde, M.; Gatz, C.; Tholl, D.
Herbivore-induced and floral homoterpene volatiles are biosynthesized by a single P450 enzyme (CYP82G1) in Arabidopsis (2010), Proc. Natl. Acad. Sci. USA, 107, 21205-21210 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Saccharomyces cerevisiae	Arabidopsis thaliana

Crystallization (Commentary)

Crystallization (Comment)	Organism
modeling and substrate docking support an oxidative bond cleavage of the alcohol substrate via syn-elimination of the polar head, together with an allylic C-5 hydrogen atom	Arabidopsis thaliana

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0018	-	(3S,6E)-nerolidol	pH not specified in the publication, temperature not specified in the publication	Arabidopsis thaliana

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	Q9LSF8	enzyme catalyzes reactions of EC 1.14.14.58 and of EC 1.14.14.59	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	highly coordinated herbivore-induced expression with geranyllinalool synthase in leaves	Arabidopsis thaliana	-
stem	constitutively expressed	Arabidopsis thaliana	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(3S,6E)-nerolidol + [reduced NADPH-hemoprotein reductase] + O2	-	Arabidopsis thaliana	(3E)-4,8-dimethylnona-1,3,7-triene + [oxidized NADPH-hemoprotein reductase] + but-3-en-2-one + 2 H2O	-	?

Synonyms

Synonyms	Comment	Organism
At3g25180	-	Arabidopsis thaliana
CYP82G1	-	Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.2	-	(3S,6E)-nerolidol	pH not specified in the publication, temperature not specified in the publication	Arabidopsis thaliana

Cofactor

Cofactor	Comment	Organism	Structure
cytochrome P450	-	Arabidopsis thaliana

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
110	-	(3S,6E)-nerolidol	pH not specified in the publication, temperature not specified in the publication	Arabidopsis thaliana

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Release 2023.1 (January 2023)