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Literature summary for 1.14.14.58 extracted from
- In planta variation of volatile biosynthesis an alternative biosynthetic route to the formation of the pathogen-induced volatile homoterpene DMNT via triterpene degradation in Arabidopsis roots (2015), Plant Cell, 27, 874-890 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene CYP705A1, the full-length CYP705A1 transcript is fused to the CaMV 35S promoter for ectopic expression in the cyp705a1-1 mutant background | Arabidopsis thaliana |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of the cyp705a1-1 null mutant, the mutant is complemented with a full-length CYP705A1 cDNA in C-terminal fusion to enhanced yellow fluorescent protein (eYFP) | Arabidopsis thaliana |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| clotrimazole | - |
Arabidopsis thaliana |  |
| miconazole | - |
Arabidopsis thaliana | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Arabidopsis thaliana | 16020 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (3S)-(E,E)-geranyllinalool + [reduced NADPH-hemoprotein reductase] + O2 | Arabidopsis thaliana | - |
(E,E)-4,8,12-trimethyltrideca-1,3,7,11-tetraene + but-1-en-3-one + [oxidized NADPH-hemoprotein reductase] + 2 H2O | - |
? | |
| arabidiol + [reduced NADPH-hemoprotein reductase] + O2 | Arabidopsis thaliana | - |
(3E)-4,8-dimethylnona-1,3,7-triene + ? + [oxidized NADPH-hemoprotein reductase] + 2 H2O | - |
? | |
| additional information | Arabidopsis thaliana | CYP705A1 cleaves the prenyl side chain of arabidiol to produce (E)-4,8-dimethyl-1,3,7-nonatriene, DMNT, and a nonvolatile C19-ketone derivative | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
- |
- |
| Arabidopsis thaliana | B0T7D7 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| flower | - |
Arabidopsis thaliana | - |
| leaf | - |
Arabidopsis thaliana | - |
| additional information | CYP705A1 clusters with the arabidiol synthase gene ABDS, and both genes are coexpressed constitutively in the root stele and meristematic tissue | Arabidopsis thaliana | - |
| additional information | CYP82G1 is not expressed in roots | Arabidopsis thaliana | - |
| root | - |
Arabidopsis thaliana | - |
| root meristem | - |
Arabidopsis thaliana | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (3S)-(E,E)-geranyllinalool + [reduced NADPH-hemoprotein reductase] + O2 | - |
Arabidopsis thaliana | (E,E)-4,8,12-trimethyltrideca-1,3,7,11-tetraene + but-1-en-3-one + [oxidized NADPH-hemoprotein reductase] + 2 H2O | - |
? | |
| (3S)-(E,E)-geranyllinalool + [reduced NADPH-hemoprotein reductase] + O2 | major substrate | Arabidopsis thaliana | (E,E)-4,8,12-trimethyltrideca-1,3,7,11-tetraene + but-1-en-3-one + [oxidized NADPH-hemoprotein reductase] + 2 H2O | - |
? | |
| (3S,6E)-nerolidol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Arabidopsis thaliana | (3E)-4,8-dimethylnona-1,3,7-triene + but-1-en-3-one + [oxidized NADPH-hemoprotein reductase] + 2 H2O | - |
? | |
| arabidiol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Arabidopsis thaliana | (3E)-4,8-dimethylnona-1,3,7-triene + ? + [oxidized NADPH-hemoprotein reductase] + 2 H2O | - |
? | |
| additional information | CYP705A1 cleaves the prenyl side chain of arabidiol to produce (E)-4,8-dimethyl-1,3,7-nonatriene, DMNT, and a nonvolatile C19-ketone derivative | Arabidopsis thaliana | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CYP82G1 | - |
Arabidopsis thaliana |
| TMTT synthase | - |
Arabidopsis thaliana |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome P450 | - |
Arabidopsis thaliana |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Arabidopsis thaliana | CYP705A1 is transiently induced in a jasmonate-dependent manner by infection with the root rot oomycete pathogen Pythium irregulare. DMNT is emitted from Arabidopsis thaliana roots upon infection with Pythium irregulare or treatment with jasmonic acid. ProCYP705A1:CYP705A1-eYFP lines, accumulation of the full-length CYP705A1 transcript is detected upon JA treatment, while only basal transcript levels of the gene are observed in ProCaMV35S-CYP705A1 lines with no jasmonate-dependent increase of transcript abundance | up |
| Arabidopsis thaliana | CYP82G1 is induced in Arabidopsis thaliana leaves upon insect feeding damage | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | CYP705A1 is a member of the Brassicaceae-specific CYP705 family | Arabidopsis thaliana |
| malfunction | the null mutant cyp82g1-1 is not impaired in root-specific DMNT biosynthesis, indicating that enzyme Cyp82g1 is not involved in the formation of DMNT in Arabidopsis thaliana roots | Arabidopsis thaliana |
| metabolism | enzyme CYP705A1 is involved in DMNT biosynthesis in Arabidopsis thaliana roots. The formation of (E)-4,8-dimethyl-1,3,7-nonatriene, DMNT, and (E,E)-4,8,12-trimethyl-1,3,7,11-tridecatetraene, TMTT, in leaf or flower tissues usually occurs by the oxidative breakdown of (E)-nerolidol or (E,E)-geranyl linalool. Arabidiol Is the precursor in DMNT biosynthesis in Arabidopsis thaliana roots, it can be degraded by CYP705A1 to a C19 ketone product (14-apo-arabidiol). Role of the DMNT biosynthetic pathway in resistance against Pythium irregulare | Arabidopsis thaliana |
| physiological function | enzyme CYP82G1 produces (3E)-4,8-dimethylnona-1,3,7-triene, DMNT, and (E,E)-4,8,12-trimethyltrideca-1,3,7,11-tetraene, TMTT, in vitro but functions as a TMTT synthase in planta because of the presence of (E,E)-geranyllinalool but not of (E)-nerolidol in Arabidopsis thaliana leaves | Arabidopsis thaliana |
| physiological function | while Arabidopsis thaliana and other angiosperms are known to produce the homoterpene (E)-4,8-dimethyl-1,3,7-nonatriene, DMNT, or its C16-analogue (E,E)-4,8,12-trimethyl-1,3,7,11-tridecatetraene, TMTT, by the breakdown of sesquiterpene and diterpene tertiary alcohols in aboveground tissues, Arabidopsis thaliana roots biosynthesize DMNT by the degradation of the C30 triterpene diol, arabidiol. The reaction is catalyzed by the Brassicaceae-specific cytochrome P450 monooxygenase CYP705A1. In volatile terpene biosynthesis, irregular homo/norterpenes can arise from different biosynthetic routes in a tissue-specific manner. DMNT negatively affects pathogen Pythium irregulare oospore germination and growth | Arabidopsis thaliana |
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