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Literature summary for 1.14.14.52 extracted from

  • Mau, C.J.; Karp, F.; Ito, M.; Honda, G.; Croteau, R.B.
    A candidate cDNA clone for (-)-limonene-7-hydroxylase from Perilla frutescens (2010), Phytochemistry, 71, 373-379.
    View publication on PubMed
Search for more literature for 1.14.14.52

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, expression in Escherichia coli as a chimeric protein fused with the N-terminal membrane insertion domain of the limonene-3-hydroxylase Perilla frutescens

Protein Variants

Protein Variants Comment Organism
additional information construction of a chimeric mutant enzyme by fusion of the N-terminal membrane insertion domain of the limonene-3-hydroxylase into limonene 7-hydroxylase. The kinetically competent recombinant protein produces a mixture of C3-, C6- and C7-hydroxylated limonene derivatives with a distribution of 33%, 14% and 53%, respectively Perilla frutescens

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Perilla frutescens
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-limonene + NADPH + H+ + O2 Perilla frutescens
-
 (-)-perillyl alcohol + NADP+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Perilla frutescens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Perilla frutescens
-
peltate gland
-
 Perilla frutescens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-limonene + NADPH + H+ + O2
-
 Perilla frutescens (-)-perillyl alcohol + NADP+ + H2O
-
 ?
additional information the kinetically competent recombinant protein produces a mixture of C3-, C6- and C7-hydroxylated limonene derivatives, i.e. (-)-perillyl alcohol, (-)-trans-isopiperitenol, and (-)-trans-carveol, with a distribution of 33%, 14% and 53%, respectively, thus it performs the reaction of EC 1.14.13.47 and 1.14.13.48, overview Perilla frutescens ?
-
 ?

Synonyms

Synonyms Comment Organism
(-)-limonene-7-hydroxylase
-
 Perilla frutescens
More the enzyme is a cytochrome P450 limonene hydroxylase Perilla frutescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
 recombinant chimeric mutant enzyme Perilla frutescens

pH Range

pH Minimum pH Maximum Comment Organism
6.75 8.5 half-maximal activity at pH 6.75 and 8.5, recombinant chimeric mutant enzyme Perilla frutescens

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Perilla frutescens