Activating Compound | Comment | Organism | Structure |
---|---|---|---|
FAD | plus FMN, 0.005 mM each, activation | Perilla frutescens | |
FMN | plus FAD, 0.005 mM each, activation | Perilla frutescens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,11-Dimethyl-6H-pyrido[4,3-b]carbazole | - |
Perilla frutescens | |
clotrimazole | i.e. 1-[chloro-alpha,alpha-diphenyl]imidazole, mixed-type, weak | Perilla frutescens | |
CO | CO:O2 ratio of 9:1, photoreversible | Perilla frutescens | |
cytochrome c | strong | Perilla frutescens | |
Metyrapone | i.e. 2-methyl-1,2-di-3-pyridyl-1-propanone, moderate | Perilla frutescens | |
miconazole | i.e. 1-[2,4-dichloro-beta-([2,4-di-chlorobenzyl]oxy)phenethyl]-imidazole, mixed-type, weak | Perilla frutescens | |
additional information | no inhibition: ancymidol, imidazole, up to 5 mM | Perilla frutescens | |
NADP+ | 2 mM | Perilla frutescens | |
SKF 525A | i.e. 2-diethyl-aminoethyl-2,2-diphenylvalerate, moderate | Perilla frutescens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.021 | - |
(-)-limonene | - |
Perilla frutescens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(-)-limonene + NADPH + O2 | Perilla frutescens | one of the key reactions of oxygenated monoterpenes, perillyl aldehyde biosynthesis | perillyl alcohol + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Perilla frutescens | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(S)-limonene + [reduced NADPH-hemoprotein reductase] + O2 = (-)-perillyl alcohol + [oxidized NADPH-hemoprotein reductase] + H2O | side-chain hydroxylation, mixed-function oxygenase | Perilla frutescens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf epidermis | oil glands on abaxial surface | Perilla frutescens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0001567 | - |
- |
Perilla frutescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-limonene + NADPH + O2 | hydroxylation at the same rate as (-)-limonene | Perilla frutescens | perillyl alcohol + NADP+ + H2O | - |
? | |
(-)-limonene + NADPH + O2 | highly specific | Perilla frutescens | perillyl alcohol + NADP+ + H2O | - |
? | |
(-)-limonene + NADPH + O2 | one of the key reactions of oxygenated monoterpenes, perillyl aldehyde biosynthesis | Perilla frutescens | perillyl alcohol + NADP+ + H2O | - |
? | |
additional information | no substrates: isolimonenes, terpinolene, alpha- or beta-phellandrene, alpha- or beta-terpinene, bicyclic monoterpenes: pinene, sabinene, alpha-thujene, p-cymene, cis- or trans-p-menthane, p-menth-1-ene, i.e. 8,9-dihydrolimonene | Perilla frutescens | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
32 | - |
- |
Perilla frutescens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Perilla frutescens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.3 | 8.3 | about half-maximal activity at pH 7.3 and 8.3 | Perilla frutescens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P450 | heme-thiolate protein, 0.2-0.9 nmol per mg protein | Perilla frutescens | |
NADH | 5.3% as effective as NADPH | Perilla frutescens | |
NADPH | - |
Perilla frutescens |