Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Geobacillus stearothermophilus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-limonene + [reduced NADH-hemoprotein reductase] + O2 | Geobacillus stearothermophilus | - |
(-)-trans-carveol + [oxidized NADH-hemoprotein reductase] + H2O | - |
? | |
(S)-limonene + [reduced NADH-hemoprotein reductase] + O2 | Geobacillus stearothermophilus BR388 | - |
(-)-trans-carveol + [oxidized NADH-hemoprotein reductase] + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | O85057 | - |
- |
Geobacillus stearothermophilus BR388 | O85057 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-limonene + [reduced NADH-hemoprotein reductase] + O2 | - |
Geobacillus stearothermophilus | (-)-trans-carveol + [oxidized NADH-hemoprotein reductase] + H2O | - |
? | |
(S)-limonene + [reduced NADH-hemoprotein reductase] + O2 | - |
Geobacillus stearothermophilus BR388 | (-)-trans-carveol + [oxidized NADH-hemoprotein reductase] + H2O | - |
? | |
1-cyclohexene 1-methanol + NAD+ | 9.6% activity compared to perillyl alcohol | Geobacillus stearothermophilus | 1-cyclohexen-1-carbaldehyde + NADH + H+ | - |
? | |
1-cyclohexene 1-methanol + NAD+ | 9.6% activity compared to perillyl alcohol | Geobacillus stearothermophilus BR388 | 1-cyclohexen-1-carbaldehyde + NADH + H+ | - |
? | |
4-isopropyl benzyl alcohol + NAD+ | 42.2% activity compared to perillyl alcohol | Geobacillus stearothermophilus | 4-isopropylbenzyl aldehyde + NADH | - |
? | |
4-isopropyl benzyl alcohol + NAD+ | 42.2% activity compared to perillyl alcohol | Geobacillus stearothermophilus BR388 | 4-isopropylbenzyl aldehyde + NADH | - |
? | |
4-methyl benzyl alcohol + NAD+ | 17.1% activity compared to perillyl alcohol | Geobacillus stearothermophilus | ? + NADH | - |
? | |
benzyl alcohol + NAD+ | 3.1% activity compared to perillyl alcohol | Geobacillus stearothermophilus | benzaldehyde + NADH | - |
? | |
benzyl alcohol + NAD+ | 3.1% activity compared to perillyl alcohol | Geobacillus stearothermophilus BR388 | benzaldehyde + NADH | - |
? | |
carveol + NAD+ | 86% activity compared to perillyl alcohol | Geobacillus stearothermophilus | ? + NADH | - |
? | |
cyclohexyl methanol + NAD+ | 15.8% activity compared to perillyl alcohol | Geobacillus stearothermophilus | cyclohexylaldehyde + NADH | - |
? | |
myrtenol + NAD+ | 4.0% activity compared to perillyl alcohol | Geobacillus stearothermophilus | myrtenal + NADH | - |
? | |
perillyl alcohol + NAD+ | 100% activity | Geobacillus stearothermophilus | perillyl aldehyde + NADH | - |
r | |
perillyl alcohol + NAD+ | 100% activity | Geobacillus stearothermophilus BR388 | perillyl aldehyde + NADH | - |
r | |
perillyl aldehyde + NADH | - |
Geobacillus stearothermophilus | perillyl alcohol + NAD+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
limonene hydroxylase | - |
Geobacillus stearothermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Geobacillus stearothermophilus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 55 | about 35% activity at 30°C, about 55% activity at 37°C, about 90% activity at 40°C, 100% activity at 45°C, about 75% activity at 50°C, about 30% activity at 55°C | Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.7 | - |
- |
Geobacillus stearothermophilus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 10 | about 40% activity at pH 5.5, about 75% activity at pH 6.5, about 90% activity at pH 7.0, 100% activity at 8.0, about 75% activity at pH 9.0, about 40% activity at pH 10.0 | Geobacillus stearothermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | stimulates the hydroxylation reaction | Geobacillus stearothermophilus | |
NAD+ | stimulates the dehydrogenase reaction | Geobacillus stearothermophilus | |
NADH | stimulates the hydroxylation reaction | Geobacillus stearothermophilus |