Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,11-Dimethyl-6H-pyrido[4,3-b]-carbazole | - |
Mentha spicata | |
clotrimazole | i.e. 1-[chloro-alpha,alpha-diphenyl]imidazole, mixed-type, strong | Mentha spicata | |
CO | CO:O2 ratio of 9:1, photoreversible | Mentha spicata | |
cytochrome c | strong | Mentha spicata | |
Metyrapone | i.e. 2-methyl-1,2-di-3-pyridyl-1-propanone, moderate | Mentha spicata | |
miconazole | i.e. 1-[2,4-dichloro-beta-([2,4-di-chlorobenzyl]oxy)phenethyl]-imidazole, mixed-type, strong | Mentha spicata | |
additional information | no inhibition: ancymidol, imidazole, up to 5 mM | Mentha spicata | |
NADP+ | 2 mM | Mentha spicata | |
SKF 525A | i.e. 2-diethylaminoethyl-2,2-diphenylvalerate, moderate | Mentha spicata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
(-)-limonene | - |
Mentha spicata |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Mentha spicata | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(-)-limonene + NADPH + O2 | Mentha spicata | one of the key reactions of oxygenated monoterpenes, biosynthesis of (-)-carvone | (-)-trans-carveol + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mentha spicata | - |
spearmint | - |
Purification (Comment) | Organism |
---|---|
partial | Mentha spicata |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf epidermis | oil glands on upper and lower surface | Mentha spicata | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00068 | - |
- |
Mentha spicata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-limonene + NADPH + O2 | hydroxylation at 25% the rate of (-)-limonene | Mentha spicata | (+)-cis-carveol + NADP+ + H2O | - |
? | |
(+)-p-menth-1-ene + NADPH + O2 | i.e. (+)-8,9-dihydrolimonene, hydroxylation at 30% the rate of (-)-limonene | Mentha spicata | (+)-cis-carvotanacetol + NADP+ + H2O | - |
? | |
(-)-(4S)-limonene + NADPH + O2 | highly specific | Mentha spicata | (-)-(4R,6S)-trans-carveol + NADP+ + H2O | - |
? | |
(-)-limonene + NADPH + O2 | one of the key reactions of oxygenated monoterpenes, biosynthesis of (-)-carvone | Mentha spicata | (-)-trans-carveol + NADP+ + H2O | - |
? | |
(-)-p-menth-1-ene + NADPH + O2 | i.e. (-)-8,9-dihydrolimonene, hydroxylation at 74% the rate of (-)-limonene | Mentha spicata | (-)-trans-carvotanacetol + NADP+ + H2O | - |
? | |
additional information | no substrates: isolimonenes, terpinolene, alpha- or beta-phellandrene, alpha- or beta-terpinene, bicyclic monoterpenes: pinene, sabinene, alpha-thujene, p-cymene, cis- or trans-p-menthane, NADH can replace NADPH, but more slowly | Mentha spicata | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
32 | - |
- |
Mentha spicata |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
- |
Mentha spicata |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.9 | 7.9 | about half-maximal activity at pH 6.9 and 7.9 | Mentha spicata |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P450 | average content of 0.0005 mmol/mg protein | Mentha spicata |