Cloned (Comment) | Organism |
---|---|
ligated into pET21a plasmid containing the ssuD gene, wild-type and mutants expressed in Escherichia coli BL21(DE3) super-competent cells | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
C54A | has little effect on FMN or FMNH2 binding, kcat/Km value decreases 6fold relative to wild-type | Escherichia coli |
C54S | has little effect on FMN or FMNH2 binding, kcat/Km value increases 3fold relative to wild-type. Is able to generate the C4a-(hydro)peroxyflavin, but the rate of formation is increased 10fold relative to wild-type | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0075 | - |
octanesulfonate | mutant C54S | Escherichia coli | |
0.0264 | - |
octanesulfonate | mutant C54A | Escherichia coli | |
0.044 | - |
octanesulfonate | wild-type SsuD | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
-80°C | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Cys54 in SsuD may be either directly or indirectly involved in stabilizing the C4a-(hydro)peroxyflavin intermediate formed during catalysis through hydrogen bonding interactions | Escherichia coli | ? | - |
? | |
octanesulfonate + FMNH2 + O2 | - |
Escherichia coli | octanal + FMN + sulfite + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SsuD | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.093 | - |
octanesulfonate | mutant C54A | Escherichia coli | |
0.47 | - |
octanesulfonate | mutant C54S | Escherichia coli | |
0.86 | - |
octanesulfonate | wild-type SsuD | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | - |
Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.3 | - |
octanesulfonate | mutant C54A | Escherichia coli | |
20 | - |
octanesulfonate | wild-type SsuD | Escherichia coli | |
63.3 | - |
octanesulfonate | mutant C54S | Escherichia coli |